Anti-WWC2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q6AWC2

Core Product Specifications and Parameters

Biological Background: WWC2 Function and Localization

• Protein WWC2, also known as BH-3-only member B or WW domain-containing protein 2, is encoded by the WWC2 gene (formerly BOMB) in humans. It is a member of the WW domain-containing protein family, characterized by coiled-coil regions and multiple WW domains.
• It functions as a key regulator of the Hippo signaling pathway (Salvador-Warts-Hippo pathway). WWC2 enhances the phosphorylation of large tumor suppressor kinase 1 (LATS1) and Yes-associated protein 1 (YAP1), leading to suppressed YAP1 transcriptional activity and negative regulation of cell proliferation and organ growth.
• Subcellularly, WWC2 localizes predominantly to the cytoplasm and cytosol, consistent with its role in cytoplasmic signaling cascades.
• The protein is annotated as a phosphoprotein, and its activity is likely modulated by phosphorylation events. Alternative splicing generates multiple isoforms, adding functional diversity.
• As a transcriptional repressor, WWC2 inhibits the oncogenic co-activator YAP1, thereby curbing cell proliferation. This places it as a potential tumor-suppressive factor within the Hippo pathway.
• The antibody’s immunogen, spanning residues 160–430, includes regions essential for protein-protein interactions, making it suitable for detecting endogenous WWC2 in complex samples.

Experimental Guidance and Technical Tips

• The immunogen was designed as a recombinant fragment (aa 160–430) of human WWC2; consider that this polyclonal antibody may recognize multiple isoforms and orthologs due to conserved epitopes.
• For Western blot, note the predicted molecular weight of ~134 kDa; use appropriate gel percentages, transfer conditions, and extended blocking to minimize background. Validate with positive and negative control lysates from human, mouse, or rat cells.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval (e.g., citrate buffer, heat-mediated) is critical. Titrate the antibody and include isotype controls to confirm specificity in tissue arrays.
• Reported cross-reactivity with mouse and rat WWC2 suggests high sequence conservation; nevertheless, verify detection in relevant species by comparing with human samples.
• For ELISA, employ an indirect setup with coated recombinant antigen or capture antibody. Optimize coating concentrations and detection reagents to achieve a linear standard curve.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a centralized platform for IVD raw-material procurement—covering validated antibody pairs, monoclonal and polyclonal antibodies, antigens, and ancillary reagents. Our Anti-WWC2 Rabbit Polyclonal Antibody supports investigations into Hippo pathway signaling and cell proliferation control, enabling assay development and kit production. Contact us to explore bulk ordering, custom specifications, or sourcing for rare target raw materials.