Anti-WWC1 (KIBRA) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8IX03

Core Product Specifications and Parameters

Biological Background: WWC1 (KIBRA) Function and Localization

• WWC1, also known as KIBRA (Kidney and brain protein), is a WW domain-containing protein that functions as a regulator of the Hippo signaling pathway.
• It acts as a negative regulator of cell proliferation and organ growth by enhancing phosphorylation of LATS1 and YAP1, thereby suppressing YAP1 transcriptional activity. Related references: PMID:24682284
• Synergizes with NF2 to induce phosphorylation of LATS1 and LATS2, further modulating Hippo pathway signaling. Related references: PMID:20159598
• Functions as a transcriptional coactivator of estrogen receptor alpha (ESR1), facilitating DYNLL1-mediated transactivation. Related references: PMID:16684779
• Regulates collagen-stimulated activation of the ERK/MAPK cascade, influencing cell migration and signaling. Related references: PMID:18190796
• Plays a role in podocyte directional migration, contributing to kidney filtration barrier maintenance. Related references: PMID:18596123
• Involved in cognitive functions and memory performance, potentially through regulating AMPA receptor trafficking. Related references: PMID:18672031
• Exhibits a broad subcellular distribution including cytoplasm, nucleus, and ruffle membranes, with tissue expression in brain, kidney, heart, colon, and mammary epithelial cells.

Experimental Guidance and Technical Tips

• For western blot (WB), note the predicted molecular weight of 125 kDa; use an appropriate gel percentage and transfer conditions optimized for high-molecular-weight proteins.
• For immunohistochemistry (IHC-P), consider using positive control tissues such as brain cortex or kidney sections, where WWC1 is endogenously expressed.
• For ELISA, the recombinant protein immunogen can serve as a positive control; ensure specificity for the target under the assay conditions.
• As a polyclonal antibody, lot-to-lot variability may occur; validate each new lot in the relevant experimental system.
• Cross-reactivity with mouse and rat has been reported, but validation in your specific sample type and assay is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for diagnostic development. This WWC1 (KIBRA) polyclonal antibody supports research in cell signaling, cancer biology, and neuroscience, and we can assist with custom antibody development and rare target sourcing to accelerate your project from concept to clinic.