Anti-WTAP Polyclonal Antibody for WB, IF/ICC, ELISA - Q15007

Core Product Specifications and Parameters

Biological Background: WTAP Function and Localization

• WTAP (Wilms tumor 1-associating protein), also known as Mum2 or hFL(2)D, is a pre-mRNA-splicing regulator and a core component of the WMM complex.
• As a component of the WMM complex, WTAP mediates N6-methyladenosine (m6A) methylation of RNAs, influencing mRNA splicing and processing efficiency. It is required for the accumulation of METTL3 and METTL14 to nuclear speckles. Related references: PMID:29507755, PMID:24316715, PMID:24407421, PMID:24981863
• WTAP functions as a mRNA splicing regulator, influencing the processing of numerous transcripts. Related references: PMID:12444081
• It regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2 (cyclin A2) mRNA, thereby enhancing transcript stability. Related references: PMID:17088532
• WTAP impairs the DNA-binding ability of the WT1 transcription factor and inhibits the expression of WT1 target genes. Related references: PMID:17095724
• Subcellularly, WTAP localizes predominantly to nuclear speckles and the nucleoplasm, with additional presence in the cytoplasm.
• The protein undergoes post-translational modifications, including acetylation and phosphorylation, and is ubiquitously expressed in human tissues.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 212-355 in the central region of human WTAP; the antibody may recognize multiple WTAP isoforms if the epitope is conserved.
• For Western blotting, expect a band near the predicted molecular weight of 44 kDa. Validate in appropriate cell lines or tissue lysates expressing WTAP.
• In immunofluorescence and immunocytochemistry, WTAP should exhibit a punctate nuclear speckled staining pattern, consistent with its known localization.
• Cross-reactivity with mouse and rat WTAP enables studies across species; however, confirm specificity in your experimental system using appropriate controls.
• For ELISA, titrate the antibody and validate using recombinant WTAP protein or peptide to ensure optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop sourcing solution for IVD raw materials. This anti-WTAP antibody supports research into RNA modifications, splicing regulation, and cell cycle control. Leverage CamelBio's supply capabilities, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and efficient sourcing for rare-target raw materials, to streamline your development workflows from concept to clinic.