Anti-WTAP Monoclonal Antibody for WB, IHC-P, ELISA - Q15007

Core Product Specifications and Parameters

Biological Background: WTAP Function and Localization

• Serves as an associated component of the WMM complex that catalyzes N6-methyladenosine (m6A) modification of RNAs, a mark that affects mRNA splicing and processing. Related references: PMID:29507755
• Required for the localization of METTL3 and METTL14 methyltransferases to nuclear speckles, facilitating m6A RNA methylation. Related references: PMID:24316715, PMID:24407421, PMID:24981863
• Functions as a pre-mRNA splicing regulator, influencing splice site selection. Related references: PMID:12444081
• Modulates G2/M cell-cycle progression by binding the 3' UTR of CCNA2 (cyclin A2) mRNA, thereby enhancing its stability. Related references: PMID:17088532
• Impairs WT1 DNA-binding activity, leading to suppression of WT1 target gene expression. Related references: PMID:17095724
• Localizes primarily to nuclear speckles and nucleoplasm, with minor presence in the cytoplasm.
• Post-translationally modified by phosphorylation and acetylation; subject to alternative splicing, yielding multiple isoforms.
• Expressed ubiquitously across tissues, reflecting its fundamental role in RNA processing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 212–355 of human WTAP; consider this region is C‑terminal to the protein’s core functional domains.
• For Western blotting, load appropriate positive controls (human, mouse, or rat cell lysates) and validate a band near 44 kDa; be aware of possible splice‑variant isoforms.
• In IHC‑P, nuclear speckle localization may be observed; optimize antigen retrieval and detection conditions to match this pattern.
• The recombinant protein format of the immunogen suggests compatibility with ELISA setups using WTAP fragments; validate coating and blocking conditions with your assay matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and research laboratories with one‑stop access to high‑quality IVD raw materials. Our inventory covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing—all designed to streamline your assay development. For WTAP and related epitranscriptomics targets, our team offers tailored procurement support to help you move from concept to clinic efficiently.