Anti-WT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P19544

Core Product Specifications and Parameters

Biological Background: WT1 Function and Localization

• WT1 acts as a transcription factor critical for cellular development and survival.
  Related references: PMID:7862533
• It specifically binds the DNA motif 5'-GCG(T/G)GGGCG-3', enabling gene regulation.
  Related references: PMID:17716689, PMID:25258363, PMID:7862533
• WT1 is essential for urogenital system development.
• WT1 exhibits dual roles as both a tumor suppressor and an oncogene in tumor formation.
• Isoforms lacking the KTS motif act as transcription factors; KTS-containing isoforms may regulate mRNA metabolism or splicing.
  Related references: PMID:15520190, PMID:16934801
• Isoform 1 displays lower DNA affinity and can bind RNA.
  Related references: PMID:19123921
• WT1 localizes to multiple subcellular compartments: nucleus, nucleolus, cytoplasm, nuclear speckles, and nucleoplasm.
• Expressed predominantly in kidney and a subset of hematopoietic cells, with post-translational modifications including isopeptide bond and ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-302) of human WT1, which may detect multiple isoforms; verify antigen recognition in your specific experimental context.
• For Western blotting, a band around 49 kDa is expected, but alternative splicing and post-translational modifications may produce additional bands; include appropriate controls.
• In IF/ICC, anticipate nuclear and possibly nucleolar or cytoplasmic staining depending on cell type and WT1 isoform expression; use appropriate fixation and permeabilization conditions.
• For ELISA, optimize coating and detection conditions using a known positive control.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio supplies high-quality antibodies like this anti-WT1 polyclonal antibody to support diagnostic and research programs. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether you need a single well-characterized antibody or a complete raw-material pipeline for IVD assay development, CamelBio streamlines your transition from concept to clinic.