Anti-WT1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P19544

Core Product Specifications and Parameters

Biological Background: WT1 Function and Localization

• WT1 (Wilms tumor protein 1, also known as WT33) is a transcription factor with multiple isoforms generated by alternative splicing, including the KTS insertion variants. The protein contains four C-terminal zinc-finger domains that mediate DNA binding.
• It recognizes the DNA consensus sequence 5'-GCG(T/G)GGGCG-3' and regulates target genes such as EPO, playing a critical role in urogenital system development. Related references: PMID:7862533 PMID:17716689 PMID:25258363
• WT1 exhibits context-dependent dual roles as a tumor suppressor and an oncogene in various cancers, with isoform-specific functions.
• Isoforms lacking the KTS motif function primarily as transcription factors, whereas KTS-containing isoforms may interact with mRNA and participate in mRNA metabolism or splicing. Related references: PMID:15520190 PMID:16934801 PMID:19123921
• Subcellular localization includes nucleus, nucleolus, nucleoplasm, nuclear speckles, and cytoplasm, reflecting its diverse functional roles.
• Expressed predominantly in the kidney and a subset of hematopoietic cells, consistent with its involvement in renal and hematological development and malignancies.
• Post-translational modifications include ubiquitination (Ubl conjugation) and metal ion coordination by zinc-finger domains, which are essential for DNA binding and transcriptional activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 60–250 of human WT1, covering the N-terminal regulatory domain upstream of the zinc-finger region. This design may favor recognition of specific isoforms or activation states; validate the reactivity pattern in your experimental context.
• Predicted molecular weight is approximately 49 kDa; confirm the band size by WB in relevant lysates (e.g., kidney cell lines) and consider possible post-translational modifications that may cause shifts.
• For IHC-P, antigen retrieval protocols (e.g., heat-induced epitope retrieval) should be optimized, and expression in kidney, testis, or tumor tissues can serve as positive controls.
• The antibody cross-reacts with mouse and rat WT1, allowing for translational studies in rodent models; however, validation in each species is recommended.
• For ELISA applications, pairing with a detection antibody that targets a non-overlapping epitope is advised to ensure assay specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined, one-stop sourcing solution for IVD raw materials, technical services, and consulting, accelerating your development from concept to clinic. This WT1 monoclonal antibody is part of our extensive catalog of high-quality antibodies validated for diagnostic and research applications, supporting oncology and urogenital disease studies. Beyond individual reagents, we provide validated antibody pairs, bulk ancillary materials, and custom sourcing for rare targets, ensuring your projects stay on track with reliable supply and expert support.