Anti-WSTF Rabbit Monoclonal Antibody for WB and ELISA - Q9UIG0

Core Product Specifications and Parameters

Biological Background: WSTF Function and Localization

• WSTF (Williams syndrome transcription factor), officially designated BAZ1B (bromodomain adjacent to zinc finger domain protein 1B), is an atypical tyrosine‑protein kinase encoded by the BAZ1B gene (also known as WBSCR9, WBSCR10).
• Functions as a key regulator of the DNA damage response by phosphorylating histone H2AX at Tyr‑142 (H2AXY142ph), a critical mark that differentiates apoptotic and repair pathways following genotoxic stress. Related references: PMID:19092802, PMID:19234442
• Acts as a regulatory subunit of the WICH‑1 and WICH‑5 ISWI chromatin remodeling complexes, which slide and space nucleosomes to facilitate DNA replication, transcription, and repair. Related references: PMID:11980720, PMID:28801535
• Contributes to RNA polymerase III transcription as part of the B‑WICH complex. Related references: PMID:16603771
• Mediates recruitment of the WICH‑5 complex to replication foci during S phase, coupling chromatin remodeling with DNA replication. Related references: PMID:15543136
• Localizes predominantly in the nucleus, consistent with its chromatin-associated functions.
• Ubiquitously expressed, with higher levels in heart, brain, placenta, skeletal muscle, and ovary.
• Subject to post‑translational modifications, including acetylation and phosphorylation; harbors a bromodomain, PHD‑type zinc finger, and a WAC‑type zinc finger.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N‑terminal region (aa 1‑100) of human WSTF. This region is highly conserved in mouse and rat, supporting the reported cross‑reactivity.
• For western blot, titrate antibody dilution using whole‑cell lysates from HeLa or HEK293 cells, which express moderate levels of WSTF; a band around 171 kDa is expected.
• For ELISA, the peptide immunogen can be used as a coating antigen to optimize assay conditions; background may be reduced by using a peptide‑blocking strategy.
• Validate the antibody in your specific experimental system, as endogenous expression levels vary by tissue (e.g., heart and brain show higher expression).
• Note that WSTF undergoes phosphorylation; treatment with DNA‑damaging agents may alter detection if using phospho‑specific conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop provider of IVD raw materials, serving diagnostic manufacturers and research laboratories worldwide. We supply validated monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents for targets involved in chromatin remodeling and DNA damage pathways, such as WSTF. With our extensive inventory and sourcing capabilities, we accelerate your development from concept to clinic.