Anti-WSB1 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y6I7

Core Product Specifications and Parameters

Biological Background: WSB1 Function and Localization

• WSB1 is a WD repeat and SOCS box-containing protein 1, also known as SOCS box-containing WD protein SWiP-1.
• It functions as a probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex, mediating ubiquitination and proteasomal degradation of target proteins.
• Recognizes type II iodothyronine deiodinase (DIO2) as a substrate for ubiquitination.
• Confers constitutive instability to homeodomain-interacting protein kinase 2 (HIPK2) through proteasomal degradation.
• Annotated with keywords including Ubl conjugation pathway, WD repeat, and alternative splicing, suggesting regulated expression and involvement in ubiquitin-like modifications.
• The protein has a calculated molecular weight of 47,432 Da, consistent with the observed ~47kDa band by Western blot.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 182–421, covering part of the WD repeat domain and SOCS box region, which may allow recognition of multiple WSB1 isoforms.
• For Western blot, consider a primary antibody dilution in the range of 1:500–1:2000, with detection of a band around 47 kDa; additional bands may appear due to post-translational modifications or alternative splicing products.
• In ELISA, this polyclonal antibody can be used as a capture or detection reagent; however, optimal coating concentration and blocking conditions should be determined empirically.
• Species cross-reactivity is listed as human; cross-reactivity with other species has not been reported and should be tested if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to validated IVD raw materials and tailored sourcing support. This anti-WSB1 polyclonal antibody supports studies of ubiquitin-proteasome pathways and DIO2/HIPK2 regulation, and can be integrated into diagnostic workflows for related disease markers. Beyond individual reagents, CamelBio offers comprehensive solutions including optimized antibody pairs, ancillary proteins, and bulk supply to streamline your development from concept to clinic.