Anti-WRN Rabbit Polyclonal Antibody for WB, ELISA - Q14191

Core Product Specifications and Parameters

Biological Background: WRN Function and Localization

• WRN encodes a multifunctional enzyme with magnesium and ATP-dependent 3'–5' DNA helicase activity on partially duplex substrates and 3'–5' exonuclease activity on dsDNA with a 5'-overhang (no activity on ssDNA or blunt dsDNA). Related references: PMID:9224595, PMID:9288107, PMID:9611231, PMID:11863428
• The protein preferentially binds and unwinds alternative DNA secondary structures like replication forks, Holliday junctions, and G-quadruplex DNA (e.g., d(CGG)n repeats), facilitating replication fork progression and preventing genome instability. Related references: PMID:10212265
• WRN is critical for DNA double-strand break repair after gamma-irradiation and for the formation of DNA replication focal centers; its depletion leads to chromosomal breaks and severe genome instability. Related references: PMID:9224595, PMID:9288107, PMID:9611231, PMID:33199508
• The protein localizes predominantly to the nucleus, including the nucleolus, nucleoplasm, and chromosomal regions, consistent with its DNA metabolism functions.
• WRN undergoes multiple post-translational modifications (acetylation, phosphorylation, ubiquitination) that likely regulate its helicase/exonuclease activities and protein–protein interactions.
• Mutations in WRN cause Werner syndrome, an autosomal recessive premature aging disorder characterized by genomic instability and cancer predisposition.

Experimental Guidance and Technical Tips

• The immunogen (aa 1223–1432) resides in the C‑terminal region outside the conserved helicase domain, potentially reducing cross‑reactivity with other RecQ family members.
• For Western blotting, expect a band near 162 kDa; use a high‑molecular‑weight marker and a low‑percentage gel (e.g., 6% SDS‑PAGE) for optimal separation.
• Validate antibody specificity in relevant human cell lines (e.g., HeLa, HEK293) using a knockdown control or a known positive lysate.
• In ELISA, optimize coating with the recombinant immunogen or full‑length WRN protein, and consider a competitive format for higher specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with end-to-end IVD raw-material sourcing, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-WRN polyclonal antibody is suited for studies in DNA repair, genome stability, and Werner syndrome. We also specialize in sourcing rare-target raw materials to accelerate your assay development.