Anti-WNT9A Rabbit Polyclonal Antibody for WB and ELISA - O14904

Core Product Specifications and Parameters

Biological Background: WNT9A Function and Localization

• WNT9A, also known as Protein Wnt-14, is a member of the Wnt family of secreted signaling proteins.
• It functions as a ligand for frizzled family receptors and activates the canonical Wnt/beta-catenin signaling pathway.
• During embryonic bone development, WNT9A is required for normal timing of IHH expression, chondrocyte maturation, and bone mineralization.
• It plays a redundant role in maintaining joint integrity.
• The protein is secreted into the extracellular space and associated with the extracellular matrix.
• Key post-translational features include a signal peptide, disulfide bonds, glycosylation, and lipidation, consistent with its secreted and extracellular matrix-associated nature.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 250-350 of human WNT9A, which may include conserved motifs; verify the antibody’s performance in mouse and rat samples as predicted by the cross-reactivity data.
• For WB, the predicted molecular weight is approximately 40 kDa; use appropriate molecular weight markers and validate detection in relevant cell or tissue lysates.
• In ELISA, the antibody can be used for antigen capture or detection; optimal coating concentrations and assay conditions should be determined experimentally.
• Consider performing a titration series to establish the optimal working dilution for your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this anti-WNT9A rabbit polyclonal antibody to researchers and diagnostic manufacturers focusing on Wnt signaling, skeletal development, and joint biology. As a one-stop sourcing partner for IVD raw materials, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom procurement for rare-target reagents to support your entire assay development workflow.