Anti-WNT7A Rabbit Polyclonal Antibody for WB and ELISA - O00755

Core Product Specifications and Parameters

Biological Background: WNT7A Function and Localization

• Protein Wnt-7a (WNT7A) is a secreted signaling protein that acts as a ligand for frizzled family receptors, activating the canonical Wnt/beta-catenin pathway.
• Plays a critical role in embryonic development, including dorsal-ventral limb patterning, skeletal development, and urogenital tract formation. Related references: PMID:16826533
• Required for central nervous system angiogenesis and blood-brain barrier regulation. Related references: PMID:30026314
• Essential for normal, sexually dimorphic development of Müllerian ducts and fertility in both sexes.
• Necessary for neural stem cell proliferation in the hippocampal dentate gyrus, cell cycle progression in neural progenitors, self-renewal, and neuronal differentiation.
• Localized to the secreted extracellular space and extracellular matrix.
• Exhibits tissue-specific expression in placenta, kidney, testis, uterus, fetal lung, and fetal/adult brain.
• Contains conserved disulfide bonds, undergoes glycosylation and lipidation; associated with developmental processes and linked to disease variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 101–200 of human WNT7A, which may confer specific immunoreactivity; consider sequence alignment with mouse WNT7A to anticipate cross-reactivity.
• For western blotting, the predicted molecular weight is ~39 kDa; validate in human and mouse samples from tissues known to express WNT7A, such as brain, kidney, or testis.
• ELISA applications may be suitable for detecting secreted WNT7A in biological fluids or cell culture supernatants; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing solutions, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For researchers studying Wnt signaling and developmental biology, we provide reliable access to anti-WNT7A polyclonal antibodies and complementary support materials. Our one-stop bridge streamlines procurement from concept to clinic, supporting your assay development needs.