Anti-WNT5B Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H1J7

Core Product Specifications and Parameters

Biological Background: WNT5B Function and Localization

• Protein Wnt-5b is a secreted signaling molecule that belongs to the Wnt family, which plays critical roles in embryonic development and tissue homeostasis.
• Acts as a ligand for frizzled family receptors, activating the Wnt signaling pathway to regulate cell fate, proliferation, and differentiation.
• Likely functions as a short-range signaling molecule, affecting the development of discrete tissue regions over only a few cell diameters.
• Localizes to the extracellular matrix and is secreted into the extracellular space, consistent with its role in cell-cell communication.
• Contains key structural features including a signal peptide, disulfide bonds, and glycosylation sites, and is classified as a glycoprotein and lipoprotein.
• Associated with developmental processes and the Wnt signaling pathway (Keywords: Developmental protein, Wnt signaling pathway).
• The human WNT5B gene maps to GeneID 81029 and encodes a protein with a calculated molecular weight of approximately 40 kDa (UniProt: 40,323 Da).

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide from the internal region (aa 100-200) of human WNT5B; verify reactivity in your target species and application.
• For Western blot (WB), a band around 40 kDa may be observed; consider using appropriate positive controls (e.g., human, mouse, or rat tissue lysates known to express WNT5B).
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution using positive tissue sections such as developing or adult tissues with known Wnt signaling activity.
• For ELISA, the antibody may be suitable as a coating or detection reagent; validate pairing with a compatible secondary antibody or detection system.
• Due to potential cross-reactivity with other Wnt family members, confirm specificity using knockout or overexpression models if critical.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with premium IVD raw materials, including validated polyclonal and monoclonal antibodies like this WNT5B Rabbit pAb. With expertise in developmental and signaling pathway targets, we offer reliable antibody pairs, bulk ancillary reagents, and customized sourcing for rare or specialized raw materials. Let us streamline your supply chain from discovery to clinical application.