Anti-WNT5A Rabbit Polyclonal Antibody for WB - P41221

Core Product Specifications and Parameters

Biological Background: WNT5A Function and Localization

• Protein Wnt-5a, also known as WNT5A, is a member of the Wnt family of secreted signaling proteins.
• Wnt-5a acts as a ligand for Frizzled receptors, capable of both activating and inhibiting canonical Wnt signaling depending on receptor context. With FZD4 it activates beta-catenin signaling, while with ROR2 it inhibits the canonical pathway by promoting beta-catenin degradation through a GSK3-independent mechanism (By similarity).
• Essential during embryogenesis for extension of the primary anterior-posterior axis, outgrowth of limbs and genital tubercle, and inhibition of type II collagen expression in chondrocytes, facilitating chondrogenesis (By similarity).
• Functions as a tumor suppressor by suppressing proliferation, migration, invasiveness, and clonogenicity of carcinoma cells. Related references: PMID:15735754
• Mediates motility of melanoma cells. Related references: PMID:17426020
• Secreted into the extracellular space and extracellular matrix.
• Expressed in differentiated thyroid carcinomas and detected in neonate heart and lung; expression is low or undetectable in anaplastic thyroid tumors. Related references: PMID:15735754, PMID:8288227
• A glycoprotein with a signal peptide, lipidation, and disulfide bonds; undergoes alternative splicing. Associated with Wnt signaling, development, and disease variants.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 42 kDa is expected; however, post-translational modifications such as glycosylation and palmitoylation may result in a higher observed molecular weight.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods like heat-induced epitope retrieval to enhance detection.
• For immunofluorescence (IF/ICC), permeabilization is required to detect the secreted protein, which may also localize intracellularly.
• The immunogen corresponds to amino acids 116–215; this peptide can be used as a blocking control in ELISA or for epitope competition assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. With this anti-WNT5A Rabbit pAb, CamelBio supports research into Wnt signaling, developmental biology, and oncology. For your immunoassay development needs, CamelBio offers validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD workflow.