Anti-WNT5A Monoclonal Antibody for WB, IHC-P, ELISA - P41221

Core Product Specifications and Parameters

Biological Background: WNT5A Function and Localization

• Protein Wnt-5a is a secreted ligand of the Wnt family, binding frizzled receptors and modulating canonical and non-canonical Wnt pathways.
• Plays dual roles: activates beta-catenin signaling with FZD4, but inhibits canonical Wnt via ROR2, promoting beta-catenin degradation independently of GSK3.
• Functions as a tumor suppressor by suppressing canonical Wnt signaling, reducing carcinoma cell proliferation, migration, and clonogenicity. Related references: PMID:15735754
• Mediates melanoma cell motility, highlighting a role in cell migration and invasion. Related references: PMID:17426020
• Essential for embryonic development, including anterior-posterior axis extension and limb/genital tubercle outgrowth.
• Predominantly secreted into the extracellular matrix; expressed in differentiated thyroid carcinomas, neonate heart, and lung. Related references: PMID:15735754 / PMID:8288227
• Post-translational modifications include glycosylation and lipidation; classified as a glycoprotein and lipoprotein.
• Alternative splicing generates multiple isoforms, expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to internal residues 281-380 of human WNT5A; thus this antibody is expected to detect total WNT5A protein. Validate specificity using knockout or knockdown models.
• For Western blotting, the predicted molecular weight is 42 kDa; however, due to glycosylation and lipidation, the observed band may shift. Use reducing conditions and include molecular weight markers.
• For IHC-P, optimize antigen retrieval and detection protocols, as WNT5A is a secreted protein that may localize to the extracellular matrix; include appropriate positive and negative tissue controls.
• In ELISA, consider pairing with another WNT5A antibody for sandwich format and confirm linearity of detection with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw material sourcing, offering validated antibodies, antigens, and ancillary reagents. For researchers studying Wnt signaling and developmental biology, CamelBio provides reliable monoclonal antibodies and technical support to accelerate assay development. Explore our catalog or contact us for customized procurement solutions.