Anti-WNT3A Polyclonal Antibody for IHC-P, ELISA - P56704

Core Product Specifications and Parameters

Biological Background: Wnt-3a Function and Localization

• Protein Wnt-3a is a secreted signaling protein of the Wnt family, encoded by the WNT3A gene, with alternative splicing variants.
• It acts as a ligand for Frizzled receptors, activating the canonical Wnt/β-catenin pathway and downstream TCF/LEF transcription factors. Related references: PMID:20093360 PMID:21244856 PMID:24841207
• During embryogenesis, Wnt-3a is essential for mesoderm development, somite formation, and neural tube morphogenesis.
• In adult tissues, it is moderately expressed in placenta and at low levels in lung, spleen, and prostate.
• The protein is secreted and found in the extracellular matrix and extracellular space.
• Post-translational modifications include glycosylation, lipidation, and disulfide bond formation, which are vital for its biological activity.
• Wnt-3a supports the self-renewal of intestinal stem cells in vitro. Related references: PMID:26902720

Experimental Guidance and Technical Tips

• The immunogen (recombinant fusion protein covering amino acids 1-352) corresponds to nearly the full-length human WNT3A, suggesting the antibody may recognize multiple linear epitopes and be suitable for detecting total WNT3A protein.
• For IHC-P on human tissues, consider employing heat-induced antigen retrieval and confirm reactivity with positive control tissues such as placenta, which shows moderate WNT3A expression.
• In ELISA applications, the antibody can be used as capture or detection reagent; pairing with a matched monoclonal antibody may enhance specificity. Validate with recombinant WNT3A protein standard.
• Cross-reactivity with rat suggests utility in rodent models; however, validate performance in the relevant tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by providing a complete range of IVD raw materials for Wnt signaling and developmental biology studies. From optimized polyclonal antibodies like this WNT3A pAb to matched antibody pairs and ancillary reagents, we offer one-stop sourcing to accelerate your assay development. Our team can also assist with sourcing rare-target raw materials, ensuring your project moves seamlessly from concept to clinic.