Anti-WNT3A Monoclonal Antibody for WB, IP, IF/ICC, IF-P, IHC-P, ELISA - P56704

Core Product Specifications and Parameters

Biological Background: WNT3A Function and Localization

• Protein Wnt-3a, encoded by the WNT3A gene, is a secreted signaling protein that acts as a ligand for frizzled family receptors (Probable).
• It activates the canonical Wnt/β-catenin pathway, leading to the activation of TCF/LEF transcription factors. Related references: PMID:20093360 PMID:21244856 PMID:24841207
• WNT3A is required for normal embryonic mesoderm development, formation of caudal somites, and neural tube morphogenesis (By similarity).
• It mediates self-renewal of stem cells at the bottom of intestinal crypts in vitro. Related references: PMID:26902720
• Subcellular location: Secreted into the extracellular space and associates with the extracellular matrix.
• Tissue specificity: Moderately expressed in placenta, with low levels in adult lung, spleen, and prostate.
• Post-translational modifications include glycosylation, lipidation, and disulfide bond formation, essential for secretion and signaling.
• Keywords: Alternative splicing, Developmental protein, Glycoprotein, Lipoprotein, Wnt signaling pathway.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide, this antibody is expected to recognize a linear epitope, making it suitable for Western blotting (WB) where proteins are denatured.
• For immunofluorescence (IF/ICC), note that WNT3A is a secreted protein; include appropriate permeabilization steps and consider using secretion inhibitors (e.g., Brefeldin A) to enhance intracellular detection.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions as formalin fixation may mask the epitope; validate staining in known positive tissues such as placenta, where WNT3A is moderately expressed.
• For ELISA and immunoprecipitation (IP), confirm antibody performance under native conditions; consider using a capture/detection antibody pair if quantitation is needed.
• Cross-reactivity with mouse and rat suggests conservation of the epitope; however, validate in your specific experimental system to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For Wnt signaling research, CamelBio offers validated antibody pairs, optimized monoclonal antibodies like this anti-WNT3A clone, and bulk ancillary reagents to support your assay development. We also specialize in sourcing rare targets—contact us for your unique project needs.