Anti-WNT2B Monoclonal Antibody for WB, ELISA - Q93097

Core Product Specifications and Parameters

Biological Background: WNT2B Function and Localization

• Protein Wnt-2b (also known as Wnt-13) is a secreted signaling protein that acts as a ligand for frizzled family receptors, activating the canonical Wnt/beta-catenin pathway.
• Functions redundantly in embryonic lung development, contributing to morphogenesis and cell fate specification.
• Two isoforms exist: isoform 1 is broadly expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon, with specific enrichment in brain regions (caudate nucleus, subthalamic nucleus, thalamus); isoform 2 is detected in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines.
• Subcellularly localized to the extracellular space and extracellular matrix after secretion; modified by glycosylation and lipidation, which are essential for its secretion and signaling activity.
• Contains conserved disulfide bonds typical of Wnt family members, contributing to its structural stability and receptor interaction.
• Classified under alternative splicing, developmental protein, and Wnt signaling pathway keywords, underscoring its roles in both embryonic and adult tissue homeostasis.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 200–300, a region likely conserved across human, mouse, and rat, consistent with the reported cross-reactivity; consider sequence alignment to predict reactivity in other species.
• For Western blot, denaturing conditions may reduce the protein to a band near the predicted 44 kDa, but post-translational modifications (glycosylation, lipidation) could shift the apparent molecular weight; validate in your specific sample system.
• In ELISA applications, the antibody may recognize native or recombinant WNT2B; proper coating and detection conditions should be optimized.
• Wnt proteins are often expressed at low levels; enrichment strategies (e.g., heparin-affinity or concentration of conditioned media) may improve signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For Wnt signaling and developmental biology projects, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to discuss how we can support your research and diagnostic development needs.