Anti-WNT2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P09544

Core Product Specifications and Parameters

Biological Background: WNT2 Function and Localization

• Protein Wnt-2 (also known as Int-1-related protein, IRP) is a member of the Wnt family of secreted signaling proteins that play key roles in development and tissue homeostasis.
• Functions as a ligand for frizzled family receptors and activates the canonical Wnt/β-catenin signaling pathway, leading to TCF/LEF-dependent transcription. (PubMed:20018874) Related references: PMID:20018874
• Acts as an upstream regulator of FGF10 expression and is essential for embryonic lung branching morphogenesis.
• Contributes to embryonic brain development by regulating the proliferation of dopaminergic neuronal precursors and neurons. (By similarity)
• Localizes to the extracellular space and extracellular matrix, consistent with its role as a secreted morphogen.
• Expressed in the thalamus of the brain, in fetal and adult lung, and in placenta.
• Contains conserved cysteine residues forming disulfide bonds, and undergoes N-glycosylation and palmitoylation, which are important for its secretion and receptor interaction.
• Keywords: Developmental protein, Disulfide bond, Extracellular matrix, Glycoprotein, Lipoprotein, Secreted, Signal, Wnt signaling pathway.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature human WNT2 protein (aa 26–360), so the antibody is expected to recognize the secreted form. Polyclonal nature may provide detection of multiple epitopes.
• For Western blotting (WB), the predicted molecular weight is ~40 kDa for the unmodified protein; however, post-translational modifications like glycosylation may result in a higher apparent molecular weight. Use appropriate reducing conditions.
• For immunohistochemistry (IHC-P), antigen retrieval (e.g., citrate buffer, pH 6.0) may improve epitope exposure. Optimize dilution and detection system for your specific tissue type.
• In ELISA, this antibody can be used as a capture or detection reagent for WNT2; validate with recombinant protein or known positive samples.
• Cross-reactivity with mouse and rat WNT2 has been reported, but sequence homology should be considered. Validate in your model system.

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CamelBio serves as a comprehensive IVD raw-material sourcing partner, connecting diagnostic manufacturers and research labs with high-quality reagents like this anti-WNT2 antibody for Wnt signaling, developmental biology, and oncology research. We offer reliable polyclonal and monoclonal antibodies, custom antibody development, and bulk supply to support your assay development and production scaling. Our supply chain ensures consistent quality and competitive lead times for your critical projects.