Anti-WNT2 Rabbit Monoclonal Antibody (WB, ELISA) - P09544

Core Product Specifications and Parameters

Biological Background: WNT2 Function and Localization

• Protein Wnt-2, also known as Int-1-like protein 1 (IRP) or INT1L1, is encoded by the WNT2 gene.
• Functions as a ligand for frizzled family receptors, activating the canonical Wnt signaling pathway that leads to TCF/LEF-mediated transcription. Related references: PMID:20018874
• Acts as an upstream regulator of FGF10 expression, playing a critical role in embryonic lung development.
• May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons.
• Primarily localized to the extracellular space and matrix; it is a secreted protein.
• Expressed in specific tissues including the thalamus region of the brain, fetal and adult lung, and placenta.
• Contains characteristic features such as disulfide bonds, glycosylation, and lipidation; classified as a developmental protein involved in Wnt signaling.

Experimental Guidance and Technical Tips

• The immunogen is an internal peptide (amino acids 231-330); the antibody is likely to recognize both native and denatured WNT2, making it suitable for WB under reducing and non-reducing conditions.
• For ELISA, consider coating with the immunogen peptide or recombinant WNT2 protein; optimize the antibody dilution in the relevant matrix.
• Cross-reactivity with mouse and rat has been reported; validate the antibody in your specific model system to confirm reactivity and specificity.
• As a secreted protein, WNT2 may be present in low abundance in cell lysates; consider using conditioned medium or enriched extracellular fractions for detection.
• The predicted molecular weight is ~40 kDa, but post-translational modifications (glycosylation, lipidation) may cause a shift in migration; include appropriate molecular weight markers and positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents tailored for Wnt signaling and developmental biology research. Our global supply network enables efficient sourcing of rare-target raw materials, supporting your transition from concept to clinic. Partner with CamelBio for customized solutions and accelerate your diagnostic development pipeline.