Anti-Wnt16 Rabbit Monoclonal Antibody for WB, ELISA - Q9UBV4

Core Product Specifications and Parameters

Biological Background: Wnt16 Function and Localization

• Protein Wnt-16 is encoded by the WNT16 gene and exists as two alternatively spliced isoforms: Wnt-16a and Wnt-16b, which differ in their tissue distribution.
• It belongs to the Wnt family of secreted signaling proteins, acting as a ligand for frizzled family receptors to activate the Wnt signaling pathway.
• Wnt-16 functions as a probable developmental protein, influencing the development of discrete tissue regions through short-range signaling over only a few cell diameters (By similarity).
• Isoform Wnt-16b is predominantly expressed in peripheral lymphoid organs (spleen, appendix, lymph nodes) and kidney, while isoform Wnt-16a shows significant expression only in the pancreas.
• The protein is secreted into the extracellular space and associated with the extracellular matrix.
• Post-translational modifications include glycosylation and lipidation, and it contains a signal peptide and multiple disulfide bonds, characteristic of Wnt family members.
• Keywords: Alternative splicing, Developmental protein, Disulfide bond, Extracellular matrix, Glycoprotein, Lipoprotein, Secreted, Wnt signaling pathway.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to amino acids 1-100 of human Wnt16 (Q9UBV4). This N-terminal region may be conserved across isoforms, but researchers should verify isoform specificity in their experimental systems.
• For western blotting (WB), consider a predicted molecular weight of ~41 kDa for the mature Wnt16 protein; however, post-translational modifications may lead to shifts in electrophoretic mobility.
• This antibody has been reported for use in WB and ELISA. For other applications, such as IHC or IF, validation by the end user is recommended.
• Cross-reactivity with mouse and rat Wnt16 is expected, but sequence alignment of the immunogen region is advisable for quantitative comparisons across species.
• Tissue expression data (pancreas, lymphoid organs, kidney) can guide positive control selection for expression studies.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing partner, CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. This anti-Wnt16 monoclonal antibody exemplifies our commitment to providing high-quality tools for Wnt signaling and developmental biology research. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, CamelBio simplifies procurement for every stage of assay development.