Anti-WNT10A Polyclonal Antibody for WB and ELISA - Q9GZT5

Core Product Specifications and Parameters

Biological Background: WNT10A Function and Localization

• WNT10A is a secreted glycoprotein that acts as a ligand for frizzled family receptors, activating the canonical Wnt/beta-catenin signaling pathway.
• It is essential for normal ectoderm development, including tooth, hair, and skin appendage formation. Related references: PMID:17847007 | PMID:28589954
• Critical for tooth development, with mutations linked to tooth agenesis and ectodermal dysplasia syndromes. Related references: PMID:17847007 | PMID:28589954 | PMID:29178643
• Plays a key role in postnatal maintenance of tongue filiform papillae and sweat ducts, promoting basal cell proliferation. Related references: PMID:28589954
• Necessary for normal hair follicle function and plantar epithelium keratinization, including expression of KRT9. Related references: PMID:28589954
• Subcellularly, WNT10A is secreted and associated with the extracellular matrix.
• The protein contains conserved disulfide bonds, is glycosylated, and is a phosphoprotein; disease-associated variants (e.g., OODD, SSPS) highlight its role in ectodermal dysplasia with palmoplantar keratoderma and hypotrichosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 158–417 of human WNT10A, a region predicted to be part of the mature protein after signal peptide cleavage. This may yield specific detection of full-length and cleaved forms.
• For Western blotting, a band around 46 kDa is expected under reducing conditions. Consider using appropriate positive controls such as cell lines expressing WNT10A (e.g., HEK293T transfected) or tissue lysates from skin or tongue.
• Cross-reactivity with mouse and rat has been reported, but sequence alignment of the immunogen region should be checked for appropriate species validation.
• For ELISA, the antibody can be used either as a capture or detection reagent; however, pairing with another clone is recommended for sandwich assays. Validate in your specific matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers IVD manufacturers and research laboratories streamlined access to critical raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise in rare target sourcing supports your Wnt pathway and developmental biology projects from bench to clinic. With this anti-WNT10A antibody, researchers gain a reliable tool for studying ectodermal dysplasia and tooth development, backed by CamelBio's commitment to quality and supply chain efficiency.