Anti-WNT1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04628

Core Product Specifications and Parameters

Biological Background: WNT1 Function and Localization

• Proto-oncogene Wnt-1, encoded by the WNT1 gene, is a secreted signaling protein also known as Int-1.
• Functions as a ligand for frizzled family receptors, activating the canonical Wnt/β-catenin pathway to promote transcriptional activation (PubMed:23499309, PubMed:23656646, PubMed:26902720). Related references: PMID:23499309 PMID:23656646 PMID:26902720
• Essential for embryonic brain and central nervous system development (By similarity).
• Plays a critical role in osteoblast function, bone development, and bone homeostasis (PubMed:23499309, PubMed:23656646). Related references: PMID:23499309 PMID:23656646
• Localizes to the extracellular matrix and is secreted into the extracellular space.
• Contains disulfide bonds, glycosylation, and lipidation modifications critical for secretion and function.
• Associated with disease variants including osteogenesis imperfecta and osteoporosis.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 231–370 of human WNT1, a region that may facilitate recognition of full-length and processed forms; consider testing in appropriate sample systems.
• For western blotting, the predicted molecular weight is 41 kDa, but post-translational modifications may cause migration shifts; validate with known positive controls.
• In immunohistochemistry and immunofluorescence, note that WNT1 is a secreted protein; staining may localize to extracellular spaces and matrix; include appropriate negative controls.
• Cross-reactivity is reported for human, mouse, and rat; when working with other species, verify reactivity independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material partner, streamlining procurement of validated antibody pairs, monoclonal and polyclonal antibodies, ancillary reagents, and rare target reagents for diagnostic and research applications. This WNT1 antibody supports investigations in developmental biology, neurobiology, and bone research. Our sourcing capabilities cover bulk supply and customized antibody development to meet your evolving project needs.