Anti-WNK3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BYP7

Core Product Specifications and Parameters

Biological Background: WNK3 Function and Localization

• WNK3 (Protein kinase lysine-deficient 3) is a serine/threonine-protein kinase encoded by the WNK3 gene (also known as KIAA1566, PRKWNK3), belonging to the WNK family of kinases.
• It is a key component of the WNK3-SPAK/OSR1 kinase cascade that regulates electrolyte homeostasis and cell volume recovery during hyperosmotic stress. Related references: PMID:16275911, PMID:16275913, PMID:16501604
• Upon cell shrinkage, WNK3 acts as a molecular crowding sensor and undergoes liquid-liquid phase separation to form membraneless condensates that concentrate its substrates. Related references: PMID:36318922
• Within these condensates, WNK3 phosphorylates and activates downstream kinases OXSR1/OSR1 and STK39/SPAK, which in turn modulate ion cotransporters (NKCCs, NCC, and KCCs) to control ion influx/efflux. Related references: PMID:22989884, PMID:16275911, PMID:16275913, PMID:16357011
• WNK3 localizes to the cytoplasm and displays tissue-specific expression, with high levels in brain, lung, kidney, liver, and pancreas, and distinct isoforms in brain (isoform 1) and kidney (isoform 3).
• Beyond its kinase activity, WNK3 serves as a scaffold protein that negatively regulates membrane localization of transporters such as KCNJ1 and SLC26A9, and enhances calcium influx through TRPV5/TRPV6. Related references: PMID:16357011, PMID:17673510, PMID:18768590

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 500–600 of human WNK3, providing a targeted region for antibody generation; consider verifying specificity using knockout or knockdown controls.
• For Western blotting, note that WNK3 has a predicted molecular weight of ~198 kDa; optimize transfer conditions for high-molecular-weight proteins (e.g., lower methanol in transfer buffer, longer transfer times).
• In immunofluorescence/ICC experiments, validate fixation and permeabilization protocols, as WNK3 is a cytoplasmic protein; include appropriate controls to confirm subcellular localization.
• For ELISA applications, assess the antibody's performance as both capture and detection reagent; cross-reactivity with mouse and rat WNK3 suggests conserved epitopes, but always verify in the relevant species context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop provider of IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. Our WNK3 rabbit polyclonal antibody aids in the study of electrolyte balance and cell volume regulation. We supply validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development.