Anti-WNK1 Rabbit Polyclonal Antibody for WB and ELISA - Q9H4A3

Core Product Specifications and Parameters

Biological Background: WNK1 Function and Localization

• WNK1 (Lysine-deficient protein kinase 1) is a serine/threonine-protein kinase that functions as the master regulator of the WNK-SPAK/OSR1 kinase cascade, critically controlling ion cotransporter activity and systemic blood pressure (PubMed:15883153, PubMed:17190791, PubMed:31656913).
  Related references: PMID:15883153, PMID:17190791, PMID:31656913
• Under hyperosmotic stress, WNK1 acts as a molecular crowding sensor, undergoing liquid-liquid phase separation to form membraneless compartments that concentrate WNK1 with its substrates OXSR1/OSR1 and STK39/SPAK, thereby enhancing downstream phosphorylation and regulatory volume increase (PubMed:36318922).
  Related references: PMID:36318922
• Beyond its canonical kinase cascade, WNK1 exerts kinase-dependent functions in angiogenesis by activating OXSR1/OSR1 for chemotaxis and STK39/SPAK for endothelial cell proliferation (PubMed:25362046), and phosphorylates additional substrates such as SYT2, PCF11, and NEDD4L to regulate synaptic vesicle binding, mRNA export (PubMed:29196535), and membrane protein stability, respectively.
  Related references: PMID:25362046, PMID:29196535
• A kinase-defective kidney-specific isoform acts as a dominant-negative regulator of the full-length WNK1, modulating sodium and potassium balance in the distal nephron, while the kinase-independent scaffold functions of WNK1 control membrane localization of various transporters and channels such as SLC4A4, SLC26A6, TRPV4, and CFTR, and promote SGK1 activation via mTORC2 recruitment (PubMed:14645531, PubMed:36373794).
  Related references: PMID:14645531, PMID:36373794
• WNK1 is widely expressed with highest levels in testis, heart, kidney, and skeletal muscle, and shows specific presence in dorsal root ganglia and the distal/connecting tubules of the kidney, consistent with its roles in nociception and electrolyte homeostasis.
• Subcellularly, WNK1 localizes to the cytoplasm, nucleus, and mitotic spindle, and participates in actin cytoskeletal reorganization, mitotic abscission, and negative regulation of autophagy (PubMed:10660600, PubMed:21220314, PubMed:27911840).
  Related references: PMID:10660600, PMID:21220314, PMID:27911840
• Post-translational modifications include phosphorylation, ubiquitination, and glycosylation; the protein is also chloride-sensitive, with mutations linked to hereditary sensory and autonomic neuropathy type II (HSAN2) and pseudohypoaldosteronism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-210) of human WNK1, which may recognize both full-length and kidney-specific isoforms—validate detection in relevant cell or tissue lysates.
• For Western blot, a band near 250 kDa is expected; however, multiple isoforms and potential cleavage products may be observed. Consider using fresh protease inhibitors and optimized transfer conditions for high-molecular-weight proteins.
• ELISA applications may benefit from using the purified recombinant protein or peptide as a positive control to establish assay sensitivity and specificity.
• Cross-reactivity with mouse and rat has been reported; verify reactivity in your species of interest with appropriate controls (e.g., knockout lysates or blocking peptide) where possible.
• When studying phosphorylation-dependent signaling, note that this polyclonal antibody is not phospho-specific; pair with phospho-specific antibodies to monitor WNK1 activation status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers streamlined access to high-quality IVD raw materials, including validated monoclonal and polyclonal antibodies, antibody pairs, and bulk ancillary reagents. For researchers focused on ion transport, hypertension, and volume regulation pathways, our sourcing network can also support procurement of rare-target antibodies, recombinant proteins, and technical consultation—helping accelerate your assay development from concept to clinical application.