Anti-WNK1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H4A3

Core Product Specifications and Parameters

Biological Background: WNK1 Function and Localization

• WNK1 is a serine/threonine-protein kinase that serves as a critical component of the WNK1-SPAK/OSR1 kinase cascade, regulating blood pressure and ion homeostasis. Under hyperosmotic stress, it acts as a molecular crowding sensor, undergoing liquid-liquid phase separation to create a membraneless compartment that concentrates WNK1 with its substrates OXSR1/OSR1 and STK39/SPAK, promoting their phosphorylation and activation. Related references: PMID:15883153, PMID:17190791, PMID:31656913, PMID:36318922
• Activated OXSR1/OSR1 and STK39/SPAK phosphorylate and activate Na-K-Cl cotransporters (SLC12A1/NKCC2, SLC12A2/NKCC1) while inhibiting K-Cl cotransporters (SLC12A5/KCC2, SLC12A6/KCC3), thereby promoting ion influx and regulatory volume increase. Related references: PMID:16263722, PMID:21321328, PMID:19665974
• Beyond ion transport, WNK1 regulates angiogenesis in endothelial cells through activation of the SPAK/OSR1 pathway, contributing to chemotaxis and proliferation. Related references: PMID:25362046
• WNK1 phosphorylates substrates including PCF11 (regulating mRNA nuclear export) and NEDD4L, and acts as a negative regulator of autophagy. It also has kinase-independent scaffold functions, such as stabilizing the ER membrane protein complex and regulating ENaC via SGK1 activation. Related references: PMID:29196535, PMID:27911840, PMID:33964204, PMID:36373794
• WNK1 is required for mitotic abscission and may participate in actin cytoskeletal reorganization. Related references: PMID:21220314, PMID:10660600
• Widely expressed with highest levels in testis, heart, kidney, and skeletal muscle; a kidney-specific kinase-defective isoform acts as a dominant-negative regulator of the full-length kinase. Subcellularly, WNK1 localizes to the cytoplasm, nucleus, and mitotic spindle.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal amino acids 1-210 of human WNK1; this region does not overlap with the kinase domain (residues 221–491), enabling detection of both full-length and potential kinase-defective isoforms.
• For Western blot, note the large predicted molecular weight (~251 kDa); use a low-percentage SDS-PAGE gel (e.g., 6%) and wet transfer with extended time to ensure efficient transfer.
• In immunofluorescence/immunocytochemistry, WNK1 localizes to the cytoplasm, nucleus, and mitotic spindle; appropriate fixation and permeabilization should be validated for the specific cell type and compartment of interest.
• As a polyclonal antibody, lot-to-lot variability may occur; include positive (e.g., HeLa, HEK293) and negative controls in each experiment to confirm reactivity.
• For ELISA, consider a sandwich format with a validated capture antibody if quantitative detection is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories a one-stop IVD raw-material sourcing solution, supporting every stage from concept to clinic. Our anti-WNK1 polyclonal antibody is part of a broad portfolio targeting key kinases and ion transport regulators for cardiovascular and renal biomarker research. Through our supply network, we offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and rare target raw-material sourcing to accelerate your assay development.