Anti-WNK1 Monoclonal Antibody for WB - Q9H4A3

Core Product Specifications and Parameters

Biological Background: WNK1 Function and Localization

• WNK1 (Serine/threonine-protein kinase WNK1) is the core kinase of the WNK1-SPAK/OSR1 cascade, which is a critical regulator of blood pressure and cell volume; it promotes regulatory volume increase by sensing hyperosmotic stress and activating ion influx pathways. Related references: PMID:15883153, PMID:17190791, PMID:31656913
• Under hyperosmotic conditions, WNK1 functions as a molecular crowding sensor, undergoing liquid-liquid phase separation to form membraneless compartments that concentrate WNK1 with its substrates OXSR1/OSR1 and STK39/SPAK, enhancing kinase signaling. Related references: PMID:36318922
• Downstream, WNK1 phosphorylates and activates OXSR1/OSR1 and STK39/SPAK, which in turn phosphorylate ion cotransporters (SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A5/KCC2, SLC12A6/KCC3) to regulate sodium, potassium, and chloride transport, thereby controlling cell volume and blood pressure. Related references: PMID:16263722, PMID:21321328, PMID:19665974
• Beyond ion transport, WNK1 regulates angiogenesis via OXSR1/OSR1 and STK39/SPAK in endothelial cells, modulates mRNA export by phosphorylating PCF11, negatively regulates autophagy, and is required for the abscission step during mitosis. Related references: PMID:25362046, PMID:29196535, PMID:27911840, PMID:21220314
• WNK1 also acts as a scaffold protein independently of its kinase activity: it stabilizes the ER membrane protein complex component EMC2, and promotes SGK1 activation by recruiting it to mTORC2 in a chloride-dependent manner. Related references: PMID:33964204, PMID:36373794
• A kidney-specific, kinase-defective isoform of WNK1 functions as a dominant-negative regulator of the full-length kinase, playing a key role in renal sodium and potassium balance. Related references: PMID:14645531
• Subcellularly, WNK1 localizes to the cytoplasm, nucleus, and mitotic spindle, consistent with its roles in signaling, gene expression, and cell division.
• WNK1 is widely expressed, with highest levels in testis, heart, kidney, and skeletal muscle; strong expression is also seen in dorsal root ganglia and spinal cord. The kidney-specific isoform is restricted to the distal convoluted tubule and connecting tubule of the nephron.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human WNK1. This N-terminal region may be conserved; cross-reactivity with mouse is confirmed. Validate the antibody in your specific model system.
• For Western blot, note the large molecular weight (~251 kDa). Use a low-percentage (e.g., 6-8%) SDS-PAGE gel and extended transfer times (e.g., overnight at 30 V) to ensure efficient transfer of high-molecular-weight proteins.
• For ELISA applications, consider using the immunogen peptide as a positive control to assess antibody binding and establish assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to supply validated raw materials, including premium monoclonal antibodies such as this anti-WNK1 clone. For assays targeting WNK1-related hypertension, ion transport disorders, or neurological conditions, we provide one-stop access to optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare target raw materials.