Anti-WLS Rabbit Polyclonal Antibody for WB, ELISA - Q5T9L3

Core Product Specifications and Parameters

Biological Background: WLS Function and Localization

• Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism, controlling expression, subcellular location, and binding (PubMed:34587386). Related references: PMID:34587386
• Essential for anterior-posterior body axis formation during development (By similarity).
• Transmembrane protein also known as GPR177, EVI, and mig-14, acting as a key component of the Wnt signaling pathway.
• Localizes to multiple membrane compartments including Golgi apparatus, cytoplasmic vesicles, cell membrane, endoplasmic reticulum, and early endosomes.
• Annotated as a glycoprotein and developmental protein; keywords indicate potential disease variants and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 43-232 of human WLS, a region likely conserved in mouse and rat, supporting the observed cross-reactivity.
• For Western blot, a band around 62 kDa is expected; consider optimizing blocking and detection conditions for membrane-bound targets.
• ELISA detection may require validation in the relevant sample matrix due to the multi-organelle localization of WLS.
• Subcellular fractionation or enrichment of membrane fractions could improve signal in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including this anti-WLS polyclonal antibody for Wnt signaling and developmental biology studies. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary reagents, and rare target sourcing—bridging the gap from concept to clinic with reliable supply solutions.