Anti-WISP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O76076

Core Product Specifications and Parameters

Biological Background: WISP2/CCN5 Function and Localization

• WISP2, also known as CCN5, CTGF-L, or CT58, is a member of the CCN family of secreted matricellular proteins involved in cell adhesion, proliferation, and differentiation.
• The protein promotes adhesion of osteoblast cells and inhibits binding of fibrinogen to integrin receptors, thereby modulating bone turnover.
• It inhibits osteocalcin production, suggesting a regulatory role in bone matrix mineralization.
• WISP2 is secreted and functions extracellularly; it contains a signal peptide and forms disulfide bonds.
• Tissue expression is observed in primary osteoblasts, fibroblasts, ovary, testes, and heart, indicating broad but selective distribution.
• The gene undergoes alternative splicing, and the reference isoform has a molecular weight of approximately 27 kDa.
• Keywords: Alternative splicing, Cell adhesion, Disulfide bond, Secreted, Signal.

Experimental Guidance and Technical Tips

• For Western blotting under reducing conditions, a band near 27 kDa is expected; validate using osteoblast or fibroblast lysates as positive controls.
• In immunofluorescence/ICC, consider permeabilization methods to detect intracellular protein pools, as WISP2 is primarily secreted.
• For ELISA applications, assess the antibody pair suitability (capture/detection) with appropriate recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents for diagnostic development. This anti-WISP2 polyclonal antibody supports research into bone remodeling, cell adhesion, and WNT signaling pathways. Contact our team to source rare-target antibodies or bulk quantities tailored to your assay requirements.