Anti-WISP2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O76076

Core Product Specifications and Parameters

Biological Background: WISP2 (CCN5) Function and Localization

• WISP2 (CCN family member 5), also known as CTGF-L and WNT1-inducible-signaling pathway protein 2, is a matricellular protein encoded by the CCN5 gene (also CT58, CTGFL).
• It plays a role in modulating bone turnover: promotes osteoblast adhesion, inhibits fibrinogen binding to integrin receptors, and suppresses osteocalcin production.
• The protein is secreted, consistent with its involvement in cell adhesion and extracellular matrix signaling.
• Tissue expression is observed in primary osteoblasts, fibroblasts, ovary, testes, and heart.
• The protein features alternative splicing, a signal peptide, and disulfide bonds, and has a calculated molecular weight of 26.8 kDa (observed ~27 kDa).
• Related keywords: cell adhesion, reference proteome, proteomics identification, direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen (aa 164–250 of human WISP2) corresponds to a specific internal region; sequence alignment with mouse and rat orthologs may explain observed cross-reactivity.
• For Western blotting, a band near 27 kDa is expected; consider using reducing and denaturing conditions.
• In immunohistochemistry (IHC-P), test various antigen retrieval methods, as WISP2 is a secreted protein and may require optimized exposure.
• For immunofluorescence (IF/ICC), permeabilization and blocking steps are critical; validate staining in known positive tissues (e.g., osteoblasts).
• ELISA applications may be suitable for quantifying secreted WISP2 in cell culture supernatants or biological fluids; consider pairing with a capture antibody for sandwich ELISA.
• Always include appropriate positive and negative controls for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic developers and research institutes with high-quality antibodies, antigens, and ancillary reagents. For bone metabolism and extracellular matrix research, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and customized rare-target sourcing solutions to accelerate your assay development. Explore our portfolio to streamline your procurement from concept to clinic.