Anti-WIPI2 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y4P8

Core Product Specifications and Parameters

Biological Background: WIPI2 Function and Localization

• WIPI2 (WD repeat domain phosphoinositide-interacting protein 2), also known as WIPI49-like protein 2, is encoded by the WIPI2 gene and belongs to the WIPI family of autophagy-related proteins.
• It acts as a core component of the autophagy machinery, regulating the formation of autophagosomes for lysosomal degradation. Related references: PMID:20505359 PMID:28561066
• WIPI2 functions in the early steps of preautophagosomal structure formation by binding phosphatidylinositol 3-phosphate (PtdIns3P) on endoplasmic reticulum membranes and mediating ER-isolation membrane contacts. Related references: PMID:28561066 PMID:28890335
• Upon activation, WIPI2 recruits the ATG12-ATG5-ATG16L1 complex to phagophore assembly sites, which is essential for ATG8 family protein lipidation and starvation-induced autophagy. Related references: PMID:20505359 PMID:28561066
• A specific isoform (isoform 4) is additionally required for xenophagy, binding to Salmonella-containing membranes and recruiting the ATG12-ATG5-ATG16L1 complex to clear pathogenic bacteria.
• The protein localizes to the membrane of preautophagosomal structures.
• WIPI2 is ubiquitously expressed, with high levels in heart, skeletal muscle, and pancreas; its expression is frequently downregulated in pancreatic and kidney tumors.
• Keywords associated with WIPI2 include alternative splicing, autophagy, disease variant (intellectual disability), lipid-binding, phosphoprotein, and WD repeat.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant protein spanning amino acids 187-436 of human WIPI2, covering a region that may recognize multiple isoforms; consider verifying isoform specificity if needed.
• For Western blotting, a band around 49 kDa is expected; validate using positive control lysates from heart, skeletal muscle, or pancreas.
• For ELISA, a recombinant WIPI2 protein can serve as a standard; ensure appropriate blocking and incubation conditions.
• As with any polyclonal antibody, batch-to-batch variability may occur; always validate the antibody in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive one-stop sourcing platform that connects diagnostic manufacturers, CROs, and research institutions with high-quality IVD raw materials. Our portfolio includes this anti-WIPI2 polyclonal antibody, which targets a key autophagy-related protein and is suitable for studies on autophagy and related disease mechanisms. Through our extensive supply network, we also offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, accelerating your development from concept to clinic.