Anti-WIPI1 Rabbit Polyclonal Antibody for WB, ELISA - Q5MNZ9

Core Product Specifications and Parameters

Biological Background: WIPI1 Function and Localization

• Protein identity and family: WIPI1 (WD repeat domain phosphoinositide-interacting protein 1), also referred to as Atg18 protein homolog or WIPI 49 kDa, belongs to the WIPI family of WD40 repeat proteins that interact with phosphoinositides. These repeats are essential for its lipid binding and autophagic functions.
• Core autophagy function: WIPI1 is a key component of the autophagy machinery, responsible for the formation of autophagosomes that deliver cytoplasmic cargo to lysosomes for degradation. It binds phosphatidylinositol 3-phosphate (PtdIns3P) and is recruited to the endoplasmic reticulum to nucleate phagophore assembly (PubMed:15602573, PubMed:20114074, PubMed:20484055). Related references:
  • PMID:15602573
  • PMID:20114074
  • PMID:20484055
• Pathway positioning: Acting downstream of ULK1 and PI3-kinases, WIPI1 senses PtdIns3P generated on ER membranes. It then assists WIPI2 in recruiting the ATG12-ATG5-ATG16L1 complex, which directly drives the elongation of the nascent autophagosomal membrane. This function is critical for both starvation- and calcium-induced autophagy and for mitophagy (PubMed:28561066). Related references:
  • PMID:28561066
• Lipid transfer and complex formation: Together with WDR45/WIPI4, WIPI1 enhances the association of ATG2 proteins (ATG2A or ATG2B) with PI3P-rich membranes, thereby promoting lipid transfer for autophagosome expansion (PubMed:31271352). Related references:
  • PMID:31271352
• Xenophagy and antimicrobial defense: WIPI1 participates in the selective autophagy of pathogens, such as Staphylococcus aureus, by engulfing bacteria in WIPI1-positive autophagosome-like vesicles destined for lysosomal destruction (PubMed:22829830). Related references:
  • PMID:22829830
• Non-autophagic roles: Beyond canonical autophagy, WIPI1 regulates melanosome maturation and transcription of melanogenic enzymes, a process independent of starvation-induced pathways (PubMed:21317285). It may also be involved in trafficking of the mannose-6-phosphate receptor (PubMed:15020712). Related references:
  • PMID:21317285
  • PMID:15020712
• Tissue distribution and subcellular localization: WIPI1 is ubiquitously expressed, with highest levels in skeletal muscle, heart, testis, pancreas, and placenta, and is upregulated in many tumor cell lines. It localizes to the trans-Golgi network, endosomes, clathrin-coated vesicles, and preautophagosomal structures, as well as associating with the cytoskeleton.

Experimental Guidance and Technical Tips

• Immunogen design: The immunogen covers amino acids 187-446 of human WIPI1, a region containing multiple WD40 repeats. This central portion is conserved across species, which aligns with the observed cross-reactivity with mouse and rat.
• Western blotting (WB): Given the high expression of WIPI1 in muscle, heart, and testis, tissue lysates from these sources are likely suitable positive controls. The predicted molecular weight is ~49 kDa, but additional bands due to alternative splicing or post-translational modifications may appear. It is advisable to include a blocking step and validate specificity using siRNA or knockout controls.
• ELISA application: This antibody can be used in indirect ELISA formats. For sandwich ELISA development, consider pairing with a monoclonal capture antibody specific to a non-overlapping epitope. Validate with recombinant WIPI1 protein (such as the immunogen) or cell lysates from highly expressing cells.
• Cross-reactivity and species consistency: The reported cross-reactivity with human, mouse, and rat implies that the epitope(s) recognized are conserved. Nonetheless, pilot Western blots with lysates from all three species are recommended to confirm reactivity and band patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to empowering diagnostic manufacturers and researchers with a complete portfolio of IVD raw materials, from validated antibody pairs to ancillary reagents. Our WIPI1 polyclonal antibody supports autophagy research and assay development, and we offer complementary services such as custom monoclonal production and bulk reagent supply to streamline your workflow. Whether you need consistent lot-to-lot performance or assistance in scaling up, CamelBio bridges the gap between concept and clinical application.