Anti-WIPF1 Polyclonal Antibody for WB, IHC-P, ELISA - O43516

Core Product Specifications and Parameters

Biological Background: WIPF1 Function and Localization

• Identified as WAS/WASL-interacting protein family member 1 (WIPF1), also known as WASP-interacting protein (WIP) or Protein PRPL-2.
• Plays a central role in reorganizing the actin cytoskeleton by contributing to the recruitment and activation of WASL (N-WASP) via adapter proteins NCK1 and GRB2.
• Involved in the regulation of WASL subcellular localization, promoting the disassembly of stress fibers and favoring filopodia formation.
• Participates in the formation of cell ruffles, dynamic membrane protrusions associated with actin remodeling.
• Functions as an adapter that facilitates the intracellular motility of vaccinia virus by recruiting WASL.
• Subcellularly localizes to cytoplasmic vesicles, the cytoskeleton, and cell projection ruffles, consistent with its actin-regulatory role.
• Highly expressed in immune-related tissues and cells, including peripheral blood mononuclear cells, spleen, and thymus, with lower expression in heart, brain, liver, and kidney.
• Post-translationally modified by phosphorylation and methylation; belongs to the WIP family and contains conserved repeat regions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the C-terminal region (aa 400-500) of human WIPF1, potentially recognizing full-length and relevant isoforms; consider sequence alignment for cross-species reactivity.
• For western blotting, a band of approximately 51 kDa is expected; however, actual migration may vary depending on gel percentage and post-translational modifications.
• In immunohistochemistry (paraffin), standard antigen retrieval methods and antibody titration are recommended to optimize specific staining, particularly in tissues with high WIPF1 expression like spleen and thymus.
• For ELISA, the antibody can be used as a capture or detection reagent; validate concentrations and coating conditions experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs and bulk reagents. For actin cytoskeleton research and Wiskott-Aldrich syndrome studies, we offer a range of polyclonal and monoclonal antibodies, as well as ancillary proteins and peptides. Leverage our sourcing expertise to accelerate your assay development from concept to clinic.