Anti-WIF1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y5W5

Core Product Specifications and Parameters

Biological Background: WIF1 Function and Localization

• WIF1, also referred to as WIF-1, is a secreted inhibitor of the Wnt signaling pathway that binds directly to WNT ligands.
• The protein contains EGF-like domains and disulfide bonds, classifying it as a glycoprotein with a signal peptide for secretion.
• Its established roles include modulation of developmental processes, such as mesoderm segmentation.
• Subcellularly, WIF1 localizes to the extracellular space and secretory pathway.
• As a Wnt pathway antagonist, WIF1 is implicated in regulation of cell fate and proliferation.
• No additional isoforms or tissue-specific functions are reported in the current UniProt annotation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 307–377) targets the C-terminal region; consider this when designing detection or blocking experiments.
• For Western blot, a band around 42 kDa is expected; verify in target species lysates with appropriate controls.
• In immunofluorescence/ICC, fixation and permeabilization conditions may influence detection of secreted WIF1; optimize protocols using known positive samples.
• ELISA applications may require antibody titration to determine optimal coating and detection concentrations.
• Cross-reactivity with mouse and rat is reported; confirm performance in respective tissues if using these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and researchers a streamlined sourcing platform for IVD raw materials. This anti-WIF1 polyclonal antibody, along with validated antibody pairs and Wnt pathway reagents, supports your assay development from concept to clinic. Our team also assists with rare-target antibody sourcing and bulk ancillary reagent procurement to meet your project needs.