Anti-WIF1 Rabbit Monoclonal Antibody for WB, ELISA - Q9Y5W5

Core Product Specifications and Parameters

Biological Background: WIF1 Function and Localization

• WIF1 (Wnt inhibitory factor 1) is a secreted protein that antagonizes Wnt signaling by directly binding to Wnt proteins and inhibiting their activities.
• It plays a role in developmental processes, possibly including mesoderm segmentation.
• The protein is classified under the Wnt signaling pathway and acts as a developmental protein.
• WIF1 contains an EGF-like domain and features disulfide bonds and glycosylation, indicating post-translational modifications important for its structure and function.
• It is a secreted protein, consistent with its function in the extracellular space to modulate Wnt signaling.
• Keywords from UniProt include: 3D-structure, signal peptide, repeat, and glycoprotein, indicating conserved structural elements.
• The predicted molecular weight is approximately 41.5 kDa (UniProt), though the observed protein weight in this product specification is 42 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide mapping to amino acids 280-379 of human WIF1, a region near the C-terminus; consider this when designing complementary detection reagents or validating epitope specificity.
• For Western blot, the observed band is around 42 kDa; optimize reducing conditions and loading controls for accurate size determination.
• In ELISA applications, consider the coating antigen and detection system to ensure optimal signal-to-noise ratio, given the antibody's monoclonal nature.
• The antibody exhibits cross-reactivity with mouse and rat WIF1; however, validate performance in the relevant species' sample matrices before quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, from concept to clinic. This anti-WIF1 monoclonal antibody supports research into Wnt signaling pathways, developmental biology, and related disease models. Beyond single antibodies, CamelBio provides validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare targets to accelerate your diagnostic development pipeline.