Anti-WIF1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y5W5

Core Product Specifications and Parameters

Biological Background: WIF1 Function and Localization

• WIF1 (Wnt inhibitory factor 1) is a secreted protein that functions as an antagonist of Wnt signaling by directly binding to Wnt proteins and inhibiting their activities.
• It contains an N-terminal signal peptide, a WIF domain, five EGF-like domains, and potential glycosylation sites, consistent with its classification as a secreted glycoprotein.
• WIF1 may be involved in mesoderm segmentation during development.
• It is detected in the extracellular space and is predicted to form disulfide bonds, typical of cysteine-rich secreted proteins.
• The predicted molecular weight of the unprocessed precursor is approximately 42 kDa, with the mature secreted form likely being smaller after signal peptide cleavage.
• Keywords from UniProt: Secreted, Signal, EGF-like domain, Wnt signaling pathway, Developmental protein, Glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 29–220 of human WIF1, which includes the WIF domain and part of the EGF-like domains. This region is expected to be present in the mature secreted protein, making the antibody suitable for detecting the processed form.
• For Western blot, a band around 42 kDa may be observed for the full-length precursor, while the mature secreted form may migrate at a lower molecular weight. Include appropriate controls and consider validation in relevant sample systems.
• For immunohistochemistry (paraffin), antigen retrieval methods may be required to expose the epitope; optimization with known positive and negative tissues is recommended.
• ELISA applications should be optimized with recombinant WIF1 protein as a standard to determine sensitivity and specificity.
• Cross-reactivity with mouse and rat is expected based on sequence homology, but individual experimental validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, technical services, and consulting for diagnostic manufacturers. This WIF1 antibody is part of our extensive portfolio of polyclonal antibodies for research and assay development. We supply validated antibody pairs, bulk reagents, and can assist with sourcing rare targets to streamline your workflow from concept to clinic.