Anti-WFS1 Polyclonal Antibody for WB, IF/ICC, ELISA - O76024

Core Product Specifications and Parameters

Biological Background: Wolframin Function and Localization

• Wolframin is encoded by the WFS1 gene (aliases: WFS, WFRS, WFSL, CTRCT41) and is a 100 kDa transmembrane glycoprotein.
• It localizes primarily to the endoplasmic reticulum (ER) membrane and is also found in cytoplasmic and secretory vesicles.
• Wolframin plays a key role in regulating cellular calcium homeostasis by modulating the filling state of the ER Ca²⁺ store (PubMed:16989814). Related references: PMID:16989814
• It negatively regulates the ER stress response and promotes the stability of V-ATPase subunits ATP6V1A and ATP1B1 by preventing their degradation (PubMed:23035048). Related references: PMID:23035048
• Mutations in WFS1 are associated with Wolfram syndrome, characterized by diabetes insipidus, diabetes mellitus, optic atrophy, and deafness, as well as non-syndromic deafness and cataracts.
• The protein is expressed at high levels in heart, brain, placenta, lung, and pancreas, with weaker expression in liver, kidney, and skeletal muscle, and is also present in pancreatic islet and beta-cell insulinoma cells.
• Post-translational modifications include acetylation, phosphorylation, and glycosylation.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against amino acids 1–285 of human WFS1; consider validating reactivity in your target species (human, mouse, rat) and sample type.
• For Western blotting (WB), a band around 100 kDa is expected; include appropriate positive and negative controls (e.g., heart, brain, or pancreas lysates).
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize permeabilization and fixation conditions due to the ER membrane localization of Wolframin.
• ELISA protocols may require optimization of coating antigen and antibody concentration; recombinant WFS1 protein can serve as a positive control.
• Be aware that cellular stress conditions can alter WFS1 expression levels and localization, which may influence experimental outcomes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, single-channel supply of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. This anti-WFS1 polyclonal antibody supports research into ER stress, calcium signaling, and Wolfram syndrome, and can be integrated into your assay development workflow. Our one-stop sourcing model also covers rare target raw-material procurement, helping you accelerate from concept to clinic.