Anti-WFS1 Monoclonal Antibody for WB, IF-P, ELISA - O76024

Core Product Specifications and Parameters

Biological Background: WFS1 Function and Localization

• WFS1 encodes wolframin, a transmembrane glycoprotein of the endoplasmic reticulum (ER).
• Regulates cellular calcium homeostasis by modulating the filling state of the ER Ca(2+) store. Related references: PMID:16989814
• Negatively regulates the ER stress response and maintains the stability of V-ATPase subunits ATP6V1A and ATP1B1 through a proteasome-independent mechanism. Related references: PMID:23035048
• Localizes primarily to the ER membrane and is also found in cytoplasmic secretory vesicles.
• Highly expressed in heart, brain, placenta, lung, and pancreas; lower expression in liver, kidney, and skeletal muscle.
• Post-translational modifications include acetylation and phosphorylation (Acetylation, Phosphoprotein).
• Mutations in WFS1 are associated with Wolfram syndrome, as well as isolated low-frequency sensorineural hearing loss and cataract.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 70-170 of human WFS1, a region conserved in rat, consistent with the observed cross-reactivity. Confirm reactivity in your sample type.
• In Western blot, expect a band around 100 kDa. Signal is strongest in tissues with high WFS1 expression, such as heart and brain.
• For immunofluorescence, permeabilization is required to detect the ER membrane-localized target. Use ER markers as colocalization controls.
• For ELISA, this recombinant monoclonal antibody may serve as a capture or detection reagent when paired with a non-competing antibody.
• Lot-to-lot reproducibility is a key advantage of this recombinant rabbit monoclonal antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies for ER stress and metabolic disease research. This anti-WFS1 antibody is one of many critical reagents we offer to support diagnostic developers and research laboratories. Beyond catalog products, we supply bulk quantities, custom antibody development, and technical consulting to move your project efficiently from concept to clinic.