Anti-WDR81 Polyclonal Antibody for WB, ELISA - Q562E7

Core Product Specifications and Parameters

Biological Background: WDR81 Function and Localization

• WDR81 is a WD repeat-containing protein that acts as a negative regulator of the PI3 kinase complex on endosomal membranes through interaction with BECN1, modulating phosphatidylinositol 3-phosphate levels to control endosome fusion, recycling, and transport. Related references: PMID:26783301
• It facilitates the delivery of specific cargos such as BST2/tetherin from early to late endosomes, indirectly promoting their lysosomal degradation. Related references: PMID:27126989
• WDR81 participates in aggrephagy, the selective autophagic degradation of ubiquitinated protein aggregates, by regulating SQSTM1 interaction with ubiquitinated proteins and recruiting MAP1LC3C. Related references: PMID:28404643
• It may also play a role in maintaining normal mitochondrial structure and organization.
• The protein is widely expressed with highest levels in the brain, particularly in the cerebellum and corpus callosum.
• Subcellularly, WDR81 localizes to endosomal membranes (early, late, lysosomal), autophagosomes, mitochondria, and cytosol, reflecting its diverse functions in membrane trafficking and autophagy.
• The protein contains WD repeat domains and a transit peptide; alternative splicing produces multiple isoforms. Mutations in WDR81 are associated with intellectual disability and cerebellar ataxia, mental retardation, and quadrupedal locomotion (CAMRQ2).

Experimental Guidance and Technical Tips

• For Western blotting, expect a primary band near 212 kDa. Consider using brain lysates, especially from cerebellum or corpus callosum, as a rich source of WDR81.
• Due to the polyclonal nature of the antibody, additional bands from isoforms or degradation products may appear. Validate specificity by peptide blocking or knockout controls.
• In ELISA applications, ensure the coating antigen includes the immunogen region (aa 340-620) to maximize detection sensitivity.
• Both reducing and non-reducing conditions may be tested to optimize detection of the full-length protein.
• Always titrate the antibody with relevant positive and negative samples to determine optimal working dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for diagnostic manufacturers and research laboratories, supplying validated IVD raw materials including antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This anti‑WDR81 polyclonal antibody supports investigations into endosomal trafficking, autophagy, and associated neurological disorders. For bulk orders or assistance with custom antibody sourcing, please contact our team.