Anti-WDR61 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9GZS3

Core Product Specifications and Parameters

Biological Background: WDR61 Function and Localization

• WDR61 (SKIC8/REC14) is a shared subunit of the PAF1 transcriptional regulatory complex and the SKI RNA decay complex, linking transcription to mRNA quality control.
  Related references: PMID:16307923, PMID:19952111, PMID:20178742, PMID:16024656, PMID:32006463, PMID:35120588
• As part of the PAF1 complex, it associates with RNA polymerase II to promote transcriptional elongation, particularly at Hox and Wnt target genes, and is essential for the establishment of histone H2B monoubiquitination (H2BK120ub1) and H3K4/H3K79 methylation.
  Related references: PMID:16307923, PMID:19952111, PMID:20178742
• The PAF1 complex also contributes to mRNA 3′ end processing and polyadenylation; during influenza A virus infection, viral NS1 protein binds the complex to alter host transcription.
  Related references: PMID:16307923, PMID:19952111, PMID:20178742
• In the cytoplasm, WDR61 is required for the SKI complex to extract mRNAs from stalled ribosomes and deliver them to the exosome for 3′–5′ degradation, ensuring efficient mRNA surveillance and turnover.
  Related references: PMID:16024656, PMID:32006463, PMID:35120588
• WDR61 localizes to both the nucleus and cytoplasm, reflecting its dual roles in nuclear transcription and cytoplasmic RNA decay.
• The protein contains WD40 repeat domains that mediate protein–protein interactions and is subject to acetylation, which may regulate its function.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein encompassing the full-length human WDR61 (amino acids 1–305); it is expected to detect endogenous WDR61 across multiple applications.
• For western blotting (WB), a principal band at approximately 34 kDa should be observed; validate specificity using knockdown or overexpression controls.
• In immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval and antibody dilution optimization are critical; pilot experiments are recommended.
• For ELISA, the antibody can be used as either a capture or detection reagent; pairing with a matched monoclonal antibody may enhance assay performance.
• Confirmed cross-reactivity with mouse and rat WDR61 allows translational studies in rodent models; confirm reactivity in the target tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement channel for IVD raw materials, including validated antibodies, recombinant antigens, and ancillary reagents for transcription and epigenetics research. This anti-WDR61 polyclonal antibody exemplifies our commitment to delivering high-quality tools for studying PAF1/SKI complex biology. Partner with us to access rare-target antibodies, bulk reagent supply, and technical consulting that accelerates your diagnostic development from concept to clinic.