Anti-WDR6 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NNW5

Core Product Specifications and Parameters

Biological Background: WDR6 Function and Localization

• WDR6 (WD repeat-containing protein 6) functions as a key regulator of tRNA methylation.
• It complexes with methyltransferase FTSJ1 to catalyze 2'-O-ribose methylation at position 34 of tRNA anticodon loops, and is required for proper substrate positioning. Related references: PMID:32558197 PMID:33771871
• WDR6 suppresses amino acid starvation-induced autophagy. Related references: PMID:22354037
• WDR6 enhances STK11/LKB1-mediated cell growth suppression. Related references: PMID:17216128
• Subcellular localization is predominantly cytoplasmic.
• It is ubiquitously expressed across tissues.
• The protein contains WD repeat domains and is subject to acetylation; it associates with cell cycle regulation and tRNA processing.

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal region (aa 800-1000), so the antibody may recognize full-length WDR6 and potential C-terminal fragments.
• For Western blotting, the predicted molecular weight is ~122 kDa; choose an appropriate gel percentage and optimize transfer conditions.
• For immunofluorescence/ICC, consider testing fixation and permeabilization protocols optimized for cytoplasmic targets.
• Cross-reactivity is reported for human and mouse; validate in your specific mouse tissue or cell system, as sequence homology in the immunogen region is high.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop platform for IVD raw materials, technical services, and consulting—from concept to clinic. This WDR6 polyclonal antibody supports research in tRNA modification, autophagy, and cell growth pathways. Beyond validated antibody pairs and bulk ancillary reagents, we assist in sourcing rare-target raw materials and offer tailored support for diagnostic development.