Anti-WDR5 Rabbit Polyclonal Antibody for WB and ELISA - P61964

Core Product Specifications and Parameters

Biological Background: WDR5 Function and Localization

• WDR5 is a core component of histone modification complexes, contributing to H3K4 methylation and histone H4 acetylation. Related references: PMID:16600877, PMID:16829960, PMID:19103755, PMID:20018852
• It positions the N-terminus of histone H3 for efficient trimethylation at Lys-4, a key epigenetic mark for transcriptional activation. Related references: PMID:16829960, PMID:18840606
• In the MLL1/MLL complex, WDR5 facilitates methylation and dimethylation at H3K4, stimulating the methyltransferase activities of KMT2 family members and SETD1A/B. Related references: PMID:19556245, PMID:21220120, PMID:22266653
• WDR5 may also regulate osteoblast differentiation, as indicated by sequence similarity.
• The protein localizes to the nucleus, consistent with its role in chromatin regulation.
• WDR5 is subject to post-translational modifications, including acetylation and ubiquitination, and contains WD repeats for protein-protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human WDR5 protein (amino acids 1-334), potentially enabling recognition of native and denatured epitopes.
• When performing Western blot, a band near the predicted 36.6 kDa (UniProt) or 37 kDa (manufacturer specification) should be observed; include appropriate positive controls.
• For ELISA, optimization of coating antigen concentration and antibody dilution is recommended to achieve optimal signal-to-noise ratio.
• Cross-reactivity is indicated for human; verification in other species may be needed if used beyond human samples.

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