Anti-WDR5 Rabbit Monoclonal Antibody for WB, ELISA - P61964

Core Product Specifications and Parameters

Biological Background: WDR5 Function and Localization

• WDR5 (WD repeat-containing protein 5), also known as BMP2-induced 3-kb gene protein (BIG3), is a member of the WD repeat protein family encoded by the WDR5 gene.
• WDR5 is a core component of the MLL/COMPASS-like histone methyltransferase complexes, where it positions histone H3 to facilitate trimethylation at Lys-4 (H3K4me3), a hallmark of active transcription. Related references: PMID:16829960, PMID:16600877
• It contributes to H3K4 dimethylation and is essential for the methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A, and SETD1B. Related references: PMID:19556245, PMID:21220120, PMID:22266653
• As part of the NSL complex, WDR5 participates in nucleosomal histone H4 acetylation on multiple lysine residues, further linking it to chromatin regulation. Related references: PMID:19103755, PMID:20018852
• WDR5 localizes predominantly to the nucleus, consistent with its role in epigenetic regulation.
• It may regulate osteoblast differentiation, as inferred from similarity studies.
• Keywords associated with WDR5 include chromatin regulator, transcription regulation, and host-virus interaction, highlighting its multifaceted cellular roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a unique region within human WDR5 (aa 235-334), which may help reduce cross-reactivity with other WD repeat proteins.
• For Western blot, consider using whole cell lysates from human or rat cell lines; the predicted molecular weight is 37 kDa, though post-translational modifications may cause slight shifts.
• For ELISA, the antibody can be used as a capture or detection reagent; titrate to optimize performance in your specific assay system.
• Validate the antibody in your target sample system with appropriate controls, as performance may vary with experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete sourcing solution for IVD raw materials, including high-quality antibodies like this WDR5 Rabbit mAb. We support diagnostic manufacturers with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. Let our supply chain expertise accelerate your epigenetic research from concept to clinic.