Anti-WDR48 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8TAF3

Core Product Specifications and Parameters

Biological Background: WDR48 Function and Localization

• WDR48, also known as USP1-associated factor 1 (UAF1) and p80, is a WD repeat-containing protein that functions as a critical activator of deubiquitinating enzymes USP1, USP12, and USP46. Related references: PMID:18082604 PMID:19075014 PMID:26388029
• It robustly enhances USP1-mediated deubiquitination of FANCD2, an essential step in the Fanconi anemia DNA repair pathway; WDR48 increases the catalytic turnover of USP1 without altering substrate affinity. Related references: PMID:18082604 PMID:31253762 PMID:19075014 PMID:27373336
• In complex with USP12, WDR48 may act as a tumor suppressor by stabilizing PHLPP1, and it binds to the distal fingers domain of USP12 to induce structural activation. Related references: PMID:24145035 PMID:27373336 PMID:27650958
• Together with RAD51AP1, WDR48 binds both single-stranded and double-stranded DNA, stimulating RAD51-mediated homologous recombination repair. Related references: PMID:27239033 PMID:27463890 PMID:32350107 PMID:31253762
• WDR48 and ATAD5 regulate deubiquitination of monoubiquitinated PCNA, facilitating translesion synthesis, and they recruit RAD51 to stalled replication forks. Related references: PMID:20147293 PMID:31844045
• Subcellularly, WDR48 is found in the nucleus, cytoplasm, lysosome, and late endosome; it is ubiquitously expressed across tissues.
• During viral infection, WDR48 interacts with Herpesvirus saimiri TIP to trigger lysosomal vesicle formation and down-regulate T-cell receptor, and with HPV11 E1 to maintain the viral genome. Related references: PMID:12196293 PMID:18032488
• Keywords associated with WDR48 include DNA damage, DNA repair, DNA-binding, host-virus interaction, and Ubl conjugation pathway, underscoring its multifaceted roles.

Experimental Guidance and Technical Tips

• The immunogen spans residues 450–677 of human WDR48 (C‑terminal region); the antibody may recognize full‑length and certain isoforms. Validate for your specific isoform of interest.
• In Western blot, expect a band near 76 kDa. Use appropriate positive (e.g., HeLa, HEK293) and negative controls, and consider reducing conditions.
• For immunohistochemistry (paraffin), optimize antigen retrieval (e.g., citrate pH 6 or EDTA pH 9) and antibody dilution per tissue type; include known positive and negative tissue controls.
• In ELISA, pairing with a detection antibody requires individual optimization; confirming reactivity against recombinant WDR48 protein is recommended.
• The antibody cross‑reacts with human, mouse, and rat based on sequence homology of the immunogen region; verify performance if using other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to a wide range of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For DNA repair and ubiquitin pathway studies, this anti‑WDR48 polyclonal antibody provides a reliable tool, and we can assist with rare‑target sourcing and custom development to accelerate your assay pipeline.