Anti-WDR46 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15213

Core Product Specifications and Parameters

Biological Background: WDR46 Function and Localization

• WDR46 is a WD repeat-containing protein, also known as BING4, belonging to the WD repeat family that forms beta-propeller structures for protein interactions.
• It functions as a scaffold component of the nucleolar structure and is required for proper localization of DDX21 and NCL to the granular compartment of the nucleolus. Related references: PMID:23848194
• WDR46 is a constituent of the small subunit (SSU) processome, the initial precursor of the small eukaryotic ribosomal subunit, and participates in pre-rRNA maturation including folding, modifications, rearrangements, cleavage, and targeted degradation by the RNA exosome. Related references: PMID:34516797
• The protein localizes to the nucleolus within the nucleus, consistent with its role in ribosome biogenesis.
• It is identified as a phosphoprotein and has been detected in proteomic analyses.
• The molecular weight of WDR46 is approximately 68 kDa, and its structure includes multiple WD40 repeats.

Experimental Guidance and Technical Tips

• For Western blotting (WB), titration of primary antibody concentration is recommended to optimize signal-to-noise. The predicted molecular weight is 68 kDa, but post-translational modifications may affect migration.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), nuclear and nucleolar staining is expected; fixation and permeabilization conditions should be empirically determined for each sample type.
• As a polyclonal antibody, it may offer enhanced sensitivity due to recognition of multiple epitopes; however, appropriate blocking steps are crucial to minimize non-specific binding.
• Cross-reactivity with mouse and rat has been reported, suggesting conservation of the immunogen sequence; validation in the intended species model is advised.
• For ELISA applications, the antibody can serve as either capture or detection reagent following pairing optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive one-stop sourcing of IVD raw materials, supporting diagnostic manufacturers and research institutions from concept to clinic. For nucleolar and ribosome biogenesis targets like WDR46, we offer validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and expert technical consultation to streamline your assay development. Our global supply network ensures rapid access to even rare-target raw materials, enabling accelerated project timelines.