Anti-WDR45 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y484

Core Product Specifications and Parameters

Biological Background: WDR45 Function and Localization

• WD repeat domain phosphoinositide-interacting protein 4 (WDR45), also known as WIPI4, is a component of the autophagy machinery. It plays a critical role in the degradation of cytoplasmic materials by promoting autophagosome formation and lysosomal delivery. Related references: PMID:23435086, PMID:28561066
• WDR45 binds phosphatidylinositol 3-phosphate (PtdIns3P) and is recruited to PtdIns3P-containing membranes, where it regulates autophagosome size downstream of WIPI2. Related references: PMID:28561066
• Together with WIPI1, WDR45 facilitates ATG2-mediated lipid transfer by enhancing ATG2 association with PI3P-rich membranes, a key step in autophagosome biogenesis. Related references: PMID:31271352
• The WDR45 gene belongs to the WD repeat protein family and is alternatively spliced, giving rise to multiple transcript variants.
• WDR45 localizes to preautophagosomal structures and the cytoplasm, consistent with its role in early autophagosome formation.
• Mutations in WDR45 are associated with neurodegeneration with brain iron accumulation (NBIA), highlighting its importance in neuronal homeostasis.
• WDR45 is ubiquitously expressed, with highest levels in skeletal muscle and heart, and reduced expression in liver, placenta, and certain tumors (e.g., pancreatic and kidney cancers).
• The protein contains multiple WD repeats and possesses lipid-binding activity, essential for its function in membrane recognition and autophagy regulation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 35–148 of human WDR45, a region conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, the predicted molecular weight is ~40 kDa; consider using appropriate positive controls (e.g., HEK293 or HeLa cell lysates) and validate band specificity.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions to preserve autophagosomal structures; induction of autophagy (e.g., by starvation or rapamycin) may enhance signal.
• ELISA assays may require optimization of coating conditions and inclusion of known WDR45-positive and negative samples.
• As WDR45 is involved in autophagy, consider validating detection in relevant disease models or under autophagy-modulating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, supporting your journey from concept to clinic. This anti-WDR45 polyclonal antibody is a valuable tool for autophagy research and assay development, and we also supply validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents to streamline your workflow. For rare target sourcing and custom material requests, our team is ready to assist.