Anti-WDR4 Rabbit Monoclonal Antibody for WB, IHC-P, ChIP, ELISA - P57081

Core Product Specifications and Parameters

Biological Background: WDR4 Function and Localization

• WDR4 is the non-catalytic component of the METTL1-WDR4 methyltransferase complex, catalyzing N(7)-methylguanine formation in tRNAs, mRNAs, and miRNAs (PubMed:12403464, PubMed:31031083, PubMed:31031084). Related references: PMID:12403464 PMID:31031083 PMID:31031084
• As a scaffold protein, WDR4 binds tRNA substrates and directs METTL1 to methylate guanine at position 46 (m7G46) in tRNAs with a 5'-RAGGU-3' motif, stabilizing tRNA tertiary structure and protecting tRNAs from decay (PubMed:36599982, PubMed:36599985, PubMed:34352206, PubMed:34352207). Related references: PMID:36599982 PMID:36599985 PMID:34352206 PMID:34352207
• WDR4-METTL1 also methylates internal sites in a subset of mRNAs and specific miRNAs such as let-7, influencing their processing and stability (PubMed:31031084, PubMed:37379838, PubMed:31031083). Related references: PMID:31031084 PMID:37379838 PMID:31031083
• Beyond its methyltransferase function, WDR4 contributes to genome stability by localizing to DNA replication sites and regulating the endonuclease activity of FEN1 (PubMed:26751069). Related references: PMID:26751069
• WDR4 localizes primarily to the nucleus and associates with chromosomes, consistent with its roles in RNA modification and DNA replication.
• WDR4 undergoes post-translational modifications including acetylation and phosphorylation, and disease-associated variants are implicated in developmental and neurological disorders such as epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• For Western blotting, expect a specific band at approximately 45kDa. Include appropriate positive and negative controls (e.g., WDR4-overexpressing or knockout lysates) to confirm specificity.
• For IHC-P, optimize antigen retrieval conditions and validate staining patterns in known WDR4-expressing tissues. Use isotype controls to assess background.
• In ChIP assays, consider that WDR4 associates with chromatin and DNA replication sites; design primers targeting genomic regions of interest and include negative control loci.
• ELISA applications can employ the antibody for direct or capture-based detection; standardize coating concentrations and block non-specific binding.
• The immunogen spans amino acids 250–350; therefore, the antibody recognizes an internal epitope conserved across human, mouse, and rat WDR4. Verify reactivity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-WDR4 rabbit mAb, ideal for studies in RNA modification, epigenetics, and genomic stability. We serve diagnostic manufacturers and research laboratories with one-stop access to optimized antibodies, bulk ancillary reagents, and rare-target sourcing. Partner with us to streamline your procurement from concept to clinical application.