Anti-WDR12 Rabbit Polyclonal Antibody for WB and ELISA - Q9GZL7

Core Product Specifications and Parameters

Biological Background: WDR12 Function and Localization

• WDR12 (also known as YTM1) is a WD repeat-containing protein that functions as a component of the PeBoW complex, essential for ribosome biogenesis.
• The PeBoW complex is required for the maturation of 28S and 5.8S ribosomal RNAs and the subsequent formation of the 60S ribosomal subunit.
• WDR12 localizes mainly to the nucleolus and nucleoplasm within the nucleus, consistent with its role in early pre-rRNA processing steps.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or interactions during ribosome assembly.
• The protein contains multiple WD repeat domains, facilitating protein-protein interactions within the ribosome biogenesis machinery.
• WDR12 is a conserved protein across eukaryotes, highlighting its fundamental role in cell growth and proliferation through ribosome production.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-280) of human WDR12, so the antibody is expected to recognize epitopes within this domain. Consider using it to probe full-length or truncated forms.
• For Western blot, a band around 48 kDa is predicted. Optimize sample preparation and loading to clearly resolve this band, especially in nuclear-enriched lysates.
• ELISA applications may benefit from using recombinant WDR12 protein as a positive control to establish binding sensitivity.
• Cross-reactivity is reported only for human; validate in model systems (e.g., mouse, rat) if needed, as sequence homology might allow detection but should be confirmed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials. For ribosome biogenesis studies, we can source validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-WDR12 product, and bulk ancillary reagents. Our integrated services also support custom reagent procurement and rare target material sourcing, enabling seamless progression from concept to clinical application.