Anti-WDHD1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O75717

Core Product Specifications and Parameters

Biological Background: WDHD1 Function and Localization

• WDHD1, also known as acidic nucleoplasmic DNA-binding protein 1 (And-1), is a WD repeat and HMG-box DNA-binding protein that functions as a core replisome component.
• It acts as a replication initiation factor, directly binding to the CMG (CDC45-MCM-GINS) helicase complex and serving as a hub to recruit additional proteins to the replication fork.
• WDHD1 localizes to the nucleus and nucleoplasm, consistent with its role in DNA replication.
• The protein undergoes multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination (isopeptide bond), which may regulate its activity during cell cycle progression.
• As a member of the WD-repeat protein family, WDHD1 facilitates protein-protein interactions essential for replisome assembly and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 830–1129) of human WDHD1, which is well-conserved in rat, supporting the observed cross-reactivity.
• For Western blotting, the predicted molecular weight is ~126 kDa; consider using appropriate gel percentages and transfer conditions to resolve high-molecular-weight proteins.
• In immunofluorescence and immunohistochemistry, expect nuclear and nucleoplasmic staining; optimize permeabilization and antigen retrieval steps for reliable detection.
• For ELISA and other quantitative applications, validate the antibody’s linear range and sensitivity using recombinant WDHD1 protein or cell lysates from human and rat origin.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including validated antibodies like this anti-WDHD1 polyclonal antibody for DNA replication studies. Our portfolio covers antibody pairs, recombinant proteins, and ancillary reagents to support kit development and biomarker validation. For customized bulk orders or rare target sourcing, our team offers tailored supply solutions to accelerate your project from concept to clinic.