Anti-WBSCR27 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8N6F8

Core Product Specifications and Parameters

Biological Background: WBSCR27 (METTL27) Function and Localization

• The protein is officially named Methyltransferase-like protein 27 (UniProt Q8N6F8), also known as Williams-Beuren syndrome chromosomal region 27 protein (WBSCR27).
• It is encoded by the METTL27 (WBSCR27) gene located in the Williams-Beuren syndrome critical region on chromosome 7.
• Based on its sequence, it belongs to the methyltransferase-like family, though its exact enzymatic activity and biological function remain largely uncharacterized.
• It has been identified through large-scale proteomics efforts (keyword: Proteomics identification) and is part of the human reference proteome.
• The protein's predicted molecular weight is approximately 26.5 kDa (UniProt), which may run at ~27 kDa on western blots depending on conditions.
• The Williams-Beuren syndrome association highlights its possible relevance to neurodevelopmental and multisystemic features of the disorder, pending further functional validation.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human WBSCR27 (aa 1–245), so the polyclonal antibody is expected to recognize multiple epitopes on the native protein. Western blot detection at approximately 27 kDa is anticipated; however, band patterns may vary across tissues and species—validate with appropriate controls.
• For IHC-P, optimize antigen retrieval (heat-induced, pH 6 or 9) and antibody concentration using known positive tissues; cross-reactivity with mouse and rat allows translational studies.
• ELISA suitability suggests potential for detecting recombinant or endogenous protein; perform checkerboard titrations for best signal-to-noise ratio.
• Since limited functional data exists for WBSCR27/METTL27, use isogenic knockout cell lines or siRNA to confirm antibody specificity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs streamlined access to a comprehensive portfolio of IVD raw materials, covering antibodies, antigens, and ancillary reagents. This anti-WBSCR27 polyclonal antibody supports investigations into Williams-Beuren syndrome biology and methyltransferase-like protein function. For bulk orders, custom formulations, or sourcing rare targets, contact our team—we're ready to bridge your concept-to-clinic pipeline.