Anti-WBSCR22 Polyclonal Antibody for WB, IF/ICC, ELISA - O43709

Core Product Specifications and Parameters

Biological Background: WBSCR22 Function and Localization

• WBSCR22 (also known as BUD23, MERM1, or Williams-Beuren syndrome chromosomal region 22 protein) is an S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N7 position of a guanine in 18S rRNA. It requires the methyltransferase adapter protein TRM112 for full activity. Related references: PMID:25851604

• Beyond its catalytic function, WBSCR22 plays a critical role in pre-rRNA processing and 40S ribosomal subunit biogenesis/export independently of its methyltransferase activity. Related references: PMID:25851604 PMID:24086612

• It acts as a locus-specific coactivator for steroid receptors (glucocorticoid, mineralocorticoid, androgen, progesterone), facilitating transactivation. WBSCR22 is required for maintaining open chromatin at the TSC22D3/GILZ locus and for histone H3K79 dimethylation, although direct histone methyltransferase activity has not been observed in vitro. Related references: PMID:24488492

• Subcellularly, WBSCR22 localizes to the nucleoplasm and cytoplasm, with enrichment in the perinuclear region.

• Widely expressed across tissues, with high levels in heart, skeletal muscle, kidney, and lung. Protein levels are elevated in bronchial brushings and normal lung, with expression in immune cells (B cells, T cells, macrophages), and tend to be down-regulated in inflammatory lung diseases and neoplasia.

• Post-translational modifications include phosphorylation and ubiquitination (UniProt). Keywords: alternative splicing, chromatin regulator, methyltransferase, ribosome biogenesis, Williams-Beuren syndrome.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is ~32kDa. Validate with appropriate positive controls (e.g., heart, skeletal muscle, or kidney lysates) and negative controls.
• In immunofluorescence/immunocytochemistry, expect nucleoplasmic and cytoplasmic staining, potentially enriched in the perinuclear region. Co-staining with organelle markers can help confirm localization.
• The antibody can be used in ELISA following standard titration protocols. Consider testing in immunoprecipitation or chromatin immunoprecipitation (ChIP) applications given the protein’s chromatin-related roles, though these have not been validated by the supplier.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers, laboratories, and research institutes with efficient access to validated raw materials, including monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for specialized targets. For projects involving ribosome biogenesis, chromatin regulation, or Williams-Beuren syndrome, our anti-WBSCR22 antibody supports your assay development needs, and our team can assist with sourcing related detection tools to streamline your workflow from concept to clinic.